Total protein of SK-6 cells infected with Linda virus and MDBK
cells infected with BVDV-1 (strain NADL) was probed with the pestivirus E2-specific antibody 6A5.
2[micro]g/mL)and non-treated MDBK
cells and results were expressed in terms of percentage of inhibition (PI) using antilogarithm values of 50% tissue culture infectious dose per 100pL (TCI[D.
Activity against normal cells including Vero and MDBK
cells were also checked and found that MKA 17, KML 2 and SSA13 all three showed impressive IC50 values (>100 ug/ml, 56.
Briefly, nuclear lysates isolated from MDBK
cells were sonicated, and cross-linked proteins were immunoprecipitated by incubation with antibody against Sp1 (Santa Cruz Biotechology Inc.
2] and then a suspension of 50ul of MDBK
cells at a concentration of 2 x [10.
The viruses were replicated in monolayer MDBK
cells with a maintenance solution of DMEM (Gibco BRL) plus 0.
Leukocyte samples or nasal swabs (200[micro]L/well) were inoculated into preformed monolayers of MDBK
cells (24 well plate) and cultivated every 72 hours.
Cells and plasmidsThe HEK-293T and MDBK
cells werepurchased from the Cell Bank of Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco's modified minimal essential medium (DMEM) respectively supplemented with 10% fetal bovine serum (FBS) and horse serum (HS) at 37 C with 5% CO2.
rbIFN treatment on uterine epithelial and MDBK
Oral glucose tolerance test (OGTT) responses, renal function and blood pressure studies were carried out in non-diabetic and STZ-induced diabetic rats, while the in vitro effects of SBE were conducted on kidney cell lines LLC-PK1 and MDBK
(Highveld Biologicals, Lyndhurst, South Africa).
Las celulas MDBK
se mantuvieron en crecimiento por 24 horas a 37[grados]C en medio esencial minimo (MEM) ([SIGMAR.
The pestivirus strains (Italy-1/10-1 and Italy-1/10-2) detected in the lung samples of 2 calves were successfully isolated on MDBK
cells as shown by the positive results of an immunofluorescence assay by using an anti-NS3 monoclonal antibody pool (3A3, 3H4, IF2).