MDBK

AcronymDefinition
MDBKMadin-Darby Bovine Kidney Cells
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Para la obtencion de ADN viral, monocapas de celulas MDBK fueron crecidas en botellas de cultivo celular de 150 [cm.sup.2] de superficie (T150) e infectadas con las cepas de referencia y los aislamientos de campo.
Herpesvirus bovino 1 (BoHV-1): actualizacion de las cepas circulantes en Argentina
Total protein of SK-6 cells infected with Linda virus and MDBK cells infected with BVDV-1 (strain NADL) was probed with the pestivirus E2-specific antibody 6A5.
Novel pestivirus species in pigs, Austria, 2015
Different concentrations of P34 (1.0 to 5.5[micro]g[mL.sup.-1]) were added onto confluent monolayers of MDBK cells and the cellular viability was assessed after 72h of incubation by the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) method and neutral red assay as described elsewhere (MOSMANN, 1983; BORENFREUND & PUERNER, 1985).
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1/Atividade do peptideo antimicrobiano P34 contra o alfaherpesvirus bovino tipo 1
For evaluation of antiviral activity of EP1 and EP2 diverse cells (MDBK, MDCK and CRFK) were infected with the viruses (25 pl/well of viral dilution), incubated for 2h and the inoculum was removed.
In Vitro ACTIVITY OF PROPOLIS ON DOMESTIC ANIMAL VIRUSES: A REVIEW/ACTIVIDAD In Vitro DEL PROPOLEO SOBRE VIRUS DE ANIMALES DOMESTICOS: UNA REVISION/ATIVIDADE In Vitro DO PROPOLIS SOBRE VIRUS DE ANIMAIS DOMESTICOS: UMA REVISAO
Activity against normal cells including Vero and MDBK cells were also checked and found that MKA 17, KML 2 and SSA13 all three showed impressive IC50 values (>100 ug/ml, 56.0 ug/ml and 64.8 ug/ml respectively) against Vero cells and IC50 values are >100 ug/ml for the strains KMF 2, KME 1 and LSA 14 against MDBK cells (Table II).
In-vitro Antitumor Activity and Metabolic Finger Printing of the Actinomycetes Isolated from Various Ecological Niches in Pakistan
Briefly, nuclear lysates isolated from MDBK cells were sonicated, and cross-linked proteins were immunoprecipitated by incubation with antibody against Sp1 (Santa Cruz Biotechology Inc., Dallas, Texas, USA) as well as normal IgGs (Cell Signaling Technology, USA), respectively.
Characterization of the Nanog 5'-flanking region in bovine
The sera-virus mixture was incubated at 37[grados]C for 24 hours in 5% C[O.sub.2] and then a suspension of 50ul of MDBK cells at a concentration of 2 x [10.sup.4] cells/well was added.
Evaluacion serologica de herpesvirus bovino 1 y 5 en sistemas de cria en la Altillanura Colombiana
Briefly, MDBK cells were inoculated with SV56/90 strain at a multiplicity of infection of 0.1.
A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain
Monolayer cultures of Madin-Darby bovine kidney (MDBK) cells (National Bank for Industrial Microorganisms and Cell Cultures, Sofia, Bulgaria) were grown in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (Gibco BRL, Paisley, Scotland, UK), supplemented with 10 mM HEPES buffer (2-[4-(2-hydroxyethyl)-l-piperazinyl]-ethanesulfonic acid) (Merck, Germany) and antibiotics (penicillin 100 IU/ml, streptomycin 100 [micro]g/ml) in C[O.sub.2] incubator (HERAcell[R] 150, Heraeus, Germany), at 37[degrees]C/5% C[O.sub.2].
Chemical composition of Propolis Extract ACF[R] and activity against herpes simplex virus
A number of cell lines of mammalian origin i.e., BHK-21, MDCK, MDBK, HeLa, and Vero are employed in the animal virology laboratories.
Optimization of Physico-chemical Factors for Augmenting Biomass Production of Baby Hamster Kidney Cells (BHK-21) in Roller Bottle
Our results from qPCR analysis (Figure 1(a)) indicated that PPARG expression is very high in all adipose tissues, followed by rumen, Madin-Darby Bovine Kidney cell line (MDBK), and placenta with moderate-to-low mRNA expression in small intestine, beef cattle longissimus muscle, hoof corium, lung, and mammary gland.
Functional role of PPARs in ruminants: potential targets for fine-tuning metabolism during growth and lactation
Terminado el tiempo de incubacion, una suspension de 4 x [10.sup.6] celulas MDBK (Madin Darby Bovine Kidney) en MEM fue adicionada en cada una de las cavidades de la placa.
Estudio seroepidemiologico del Herpesvirus Bovino 1 (BoHV-1) en ganado destinado al sacrificio en la region del Magdalena Medio colombiano
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