MDBKMadin-Darby Bovine Kidney Cells
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Total protein of SK-6 cells infected with Linda virus and MDBK cells infected with BVDV-1 (strain NADL) was probed with the pestivirus E2-specific antibody 6A5.
2[micro]g/mL)and non-treated MDBK cells and results were expressed in terms of percentage of inhibition (PI) using antilogarithm values of 50% tissue culture infectious dose per 100pL (TCI[D.
Activity against normal cells including Vero and MDBK cells were also checked and found that MKA 17, KML 2 and SSA13 all three showed impressive IC50 values (>100 ug/ml, 56.
Briefly, nuclear lysates isolated from MDBK cells were sonicated, and cross-linked proteins were immunoprecipitated by incubation with antibody against Sp1 (Santa Cruz Biotechology Inc.
2] and then a suspension of 50ul of MDBK cells at a concentration of 2 x [10.
The viruses were replicated in monolayer MDBK cells with a maintenance solution of DMEM (Gibco BRL) plus 0.
Cells and plasmidsThe HEK-293T and MDBK cells werepurchased from the Cell Bank of Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco's modified minimal essential medium (DMEM) respectively supplemented with 10% fetal bovine serum (FBS) and horse serum (HS) at 37 C with 5% CO2.
Oral glucose tolerance test (OGTT) responses, renal function and blood pressure studies were carried out in non-diabetic and STZ-induced diabetic rats, while the in vitro effects of SBE were conducted on kidney cell lines LLC-PK1 and MDBK (Highveld Biologicals, Lyndhurst, South Africa).
Las celulas MDBK se mantuvieron en crecimiento por 24 horas a 37[grados]C en medio esencial minimo (MEM) ([SIGMAR.
The pestivirus strains (Italy-1/10-1 and Italy-1/10-2) detected in the lung samples of 2 calves were successfully isolated on MDBK cells as shown by the positive results of an immunofluorescence assay by using an anti-NS3 monoclonal antibody pool (3A3, 3H4, IF2).