The most commonly used cell lines for vaccine production are Vero, Madin-Darby canine kidney, (MDCK
This dye was tested with various samples of the human cell cultures (human breast carcinoma HBL-100, human keratinocytes HaCaT, human epithelial carcinomas A431, and primary cell cultures of human lymphocytes) as well as with canine kidney epithelial cells MDCK
. Investigation of such cell staining by PFD (precursor) and Rho813 (dye) using fluorescence microscopy allows one to find the optimal sample concentrations in various solvents (technical notes concerning this procedure have been published earlier [19-23]).
Kinetics of Viral Replication in MDCK
Cells and Chickens.
By means of light and transmission electron microscopy we present some observations on the morphology of this amoeba isolated from a case of keratitis as well as its cytopathic effect on MDCK
epithelial cell monolayers.
All microneutralization assays were performed on MDCK
cells, with cells used for a maximum of 25 passages.
To account for any cell-culture induced mutations, sialic acid (SA) binding affinities of both MDCK
cell- and embryonated hens' egg-grown stocks of Ca/CO-1, Ca/WY, Ca/CO-2, and Eq/CO were determined using a solid-phase binding assay [31, 32], with slight modification of the original protocol to equilibrate the viruses assayed to ~20,000 matrix gene copies [33, 34].
Additional inhibitory effect of tea extract on the growth of influenza A and B viruses in MDCK
En terminos generales se puede decir que tradicionalmente, se han empleado los huevos embrionados de pollo y los cultivos de celulas Madin Darby Canine Kidney (MDCK
) para el aislamiento y propagacion del virus de influenza (31).
Diante das consideracoes aqui relatadas e levandose em conta que a roma possui um potencial a ser melhor explorado como fonte de compostos antioxidantes e seus beneficios na prevencao de doencas, o presente trabalho teve por objetivos identificar os principais acidos fenolicos presentes na polpa e sementes, avaliar a atividade antioxidante in vitro de suas fracoes fenolicas, e tambem verificar a influencia da fracao de fenolicos livre da polpa na viabilidade celular em cultura de celulas da linhagem MDCK
Identification of HAX-1 as a protein that binds bile salt export protein and regulates its abundance in the apical membrane of MDCK
Detection of influenza virus by centrifugal inoculation of MDCK
cells and staining with monoclonal antibodies.
Takizawa et al (6) reported that chromosomal DNA in influenza A virus-infected MDCK
cells exhibited fragmentation by 6 h p.i.