In LPS-stimulated MEFs
, Regnase-1 and Roquin tend to control target inflammatory mRNAs in the early and late phase, respectively.
MFN2 acts as a suppressor of proliferation and may inhibit the proliferation of vascular smooth muscle cells and MEFs
., Dasgupta et al . reported that MFN2 exhibited antiproliferative properties in activated peripheral blood T cells.
The zona pellucida of the blastocysts was removed by acid-Tyrode's solution and, the zona-free blastocysts were subsequently seeded on MEF
feeder layers in a gelatin coated four well multidishes containing knockout DMEM (kDMEM; Gibco Invitrogen, USA) supplemented with 3:1 mixture of FBS (Hyclone Laboratories, USA) and knockout serum replacement (Gibco Invitrogen, USA), 1% (v/v) nonessential amino acids (NEAA; Gibco Invitrogen, USA), 0.1 mmol/L [beta]-mercaptoethanol (Gibco Invitrogen, USA), 1% (v/v) penicillinstreptomycin solution and 500 unit/mL of leukemia inhibitory factor (LIF) (Chemicon, Temecula, CA, USA), this medium was used continuously for ESCs culture [17,18].
 In MEF
, BNIP3 mediates the mitochondrial autophagy to remove ROS, preventing cell death.
In addition, there is the availability of mouse embryonic fibroblast (MEF
) lines for many strains, which may be an attractive choice for in vitro analyses while MEF
and stem cell lines for next-generation mouse populations are still under development (Suzuki et al.
and HeLa cells were transfected with scramble NC (NC), miR-93 mimics (93), NC inhibitor (IN-NC), or miR-93 inhibitor (IN-93) individually for 12 h, followed by the treatment of hypoxia for another 24 h.
In this study, the primary culture of trophoblasts was done by culturing of hatching/hatched blastocysts on feeder cells, which were either mouse embryonic fibroblasts (MEFs
) or, for the first time, porcine granulosa cells (PGCs).
hiPSCs cultured on the MEFs
feeder were differentiated using the following four protocols and hiPSCs cultured on the hMSCs feeder were differentiated using only protocol 5.
The experiments results showed that, our RFID data streams cleaning strategy method based on MEFS
had lower time complexity compared to the algorithms that adopted ID3 decision tree in the literature .
Through expert management, coordination, and utilization of transportation resources, the command recapitalized and redistributed (111) low density high demand in-theater communications assets, valued at over $65 million, enabled the Marine Corps the ability to satisfy urgent mission requirements for First and Second Marine Expeditionary Forces (MEFs
The embryonic fibroblasts (MEFs
) from these mutant mice showed increased rate of DNA breaks after treatment of aphidicolin .
Reduced expression of MARCH5 in mouse embryonic fibroblasts (MEFs
) leads to decreased MFN2 in the MAM, decreased ER-mitochondrial colocalization due to reduced tethering of the ER to the mitochondria and decreased mitochondrial calcium uptake.