MEKCMicellar Electrokinetic Chromatography
MEKCMiddle East Karting Cup (Cairo, Egypt)
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1) as pre-capillary derivatizing reagent for the s eparatio n and determination of five guanidino compounds : G, GAA, GPA, GBA, and GSA by MEKC.
A s ys temic s tudy was carried out to optimize experimental conditions for the MEKC s eparation and determination of gu anidino compounds .
Table-1: Quantitative data of guanidino compounds by MEKC using 2, 2- pyridoin as derivatizing reagent.
The pres ent pyridoin MEKC method s eparates 5 guanidino compounds within five min with ins ignificant cons umption of s olvent greater than 0.
The res ults obtained were als o compared with MEKC method us ing benzoin as derivatizing reagent [29].
5 mls olution was trans ferred to a 5 ml volumetric flas k, and the analytical procedure MEKC was followed.
The aim of this study was to evaluate the potential of MEKC both as a qualitative assay to help in identification of SU drugs in serum for rapid and accurate diagnosis of drug-induced hypoglycemia and as a quantitative technique to be used in pharmacokinetic investigations.
When the MEKC conditions (slightly modified) described by Roche et al.
Kunkel and Watzing (19), using direct injection of plasma samples for drug quantification by MEKC, found a dramatic improvement of the detection limit (to 0.
5] Nonstandard abbreviations: SU, sulfonylurea; TL, tolbutamide; CL, chlorpropamide; GP, glipizide; GL, gliclazide; GB, glibenclamide; CE, capillary electrophoresis; MEKC, micellar electrokinetic capillary chromatography; [t.