MEOH


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AcronymDefinition
MEOHMethanol
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Yellow powder (35 mg) Melting point: 314 [degrees]C; UV(MeOH) 338, 266, 214; IR (KBr) [[upsilon].sub.max] [cm.sup.-1]: 3416, 1670, 1610 1179; [.sup.1]H-NMR (500 MHz [C.sub.5][D.sub.5]N,) ([delta]ppm), (J in Hz) 7.21 (d, H each, J = 9.1, H-3'5'), 6.92 (s, H, H-3), 7.91 (d, H each, J = 9.2, H-2'6') 6.81 (d, 1H, J = 2.1, H-8), 6.75 (1H, d, J = 2.0, H-6).
The solvent was evaporated and the formed precipitate was filtered off, recrystallized from ethanol to yield white crystals in 95% yield, m.p.182-184[degrees]C [R.sub.f] = 0.31 (5% MeOH in [CHCl.sub.3]).
Antimicrobial activity tests were conducted with the agar dilution method recommended by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) on mushroom EtOH, MeOH, and DCM extracts.
The best recovery rates, up to 98-99%, for propranolol and bisoprolol were achieved with an extraction solvent consisting of MeOH or MeOH/[H.sub.2]O mixtures in different combination with or without 0.1% formic acid (FA).
Compound 1, a pale yellow oil, [[[alpha]].sup.27.sub.D] -117[degrees] (c = 0.35, MeOH), was identified as 2(R)-hydroxy-3,5-nonadiyn-2-0-[beta]-D-glucopyranoside named as bhutkesoside A, which was a new diacetylene glucoside isolated from the roots of L.
In calculations of n for the reaction between DPPH and antioxidants in MeOH, water, or buffer (pH 5.0), we used the [epsilon] value of 12000 L x [mol.sup.-1] x [cm.sup.-1] [26].
The working standard solution was prepared by diluting the mixed standard solution with MeOH to a series of proper concentrations.
HPTLC plates were used to identify the lipids PLFE and hGDNT using the mobile phases CH[Cl.sub.3]: MeOH: [H.sub.2]O (22.5: 50: 22.5 v/v) and CH[Cl.sub.3]: MeOH (90: 10 v/v), respectively.
The resulting resin-bound PMM-peptide conjugate 5 was treated with 0.5 M sodium methoxide in MeOH and repeatedly washed with a sodium-complexing solution to remove O-acetyl groups [20].
To a portion of EEFFF (1.0 g), MeOH was added and an insoluble precipitate was separated by filtration on sintered glass funnel and washed thoroughly with methanol.
For the extraction step, the authors initially evaluated ethanol (EtOH), ACN, MeOH, and 9/1 MeOH/CH[Cl.sub.3] for their ability to extract and stabilize metabolites.