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The prepared Mesua ferrea methanolic extract (MFME) was subjected for filtration and concentrated under reduced pressure, followed with freeze drying.
The MFME acute toxicity studies were preformed on normal rats as previously described by Lorke et al.
Diabetic animals were divided into three groups (n=8): Group II served as diabetic control, while group III and group IV received MFME (200 mg/kg bw) and glibenclamide (5 mg/kg bw), respectively, for a period of 4 weeks.
Then the cells were treated with MFME at a concentration range of 5-1000 g/mL and standard drug, glibenclamide (5-100 M/mL), allowed for 72 hrs incubation.
MFME extract stock solution was prepared by dissolving in DMSO and further working solution were prepared by using KRB.
The insulin releasing mechanism of MFME was ascertained in the presence of diazoxide (0.5 mM) (K+- ATP channel opener), IBMX (100 M) (phosphodiesterase inhibitor) and Glibenclamide.
Acute Toxicity Studies and Glucose Tolerance Test Acute toxicity studies of prepared MFME extract at doses of 0.25, 0.5, 1.0 and 2.0 g/kg bw were proven safe without any toxicity symptoms, behavioral changes and mortality.
Glucose tolerance test of MFME at 200 mg/kg bw dose was effectively reduced blood glucose levels than the doses of 50 and 100 mg/kg bw (Table 1).
The effect of MFME (200 mg/kg bw) on the body weight on 1, 7, 14, 21 and 28 days of study in the STZ induced diabetic rats were shown in Figure 1.
Table 1: Oral Glucose Tolerance Test of MFME at Different Doses
MFME (100 mg/kg)###81.852.5###129.573.97###103.813.18###97.074.47###88.982.72
MFME (200 mg/kg)###81.093.36###120.373.49###95.093.06###86.223.97###83.592.93
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