MGCK

AcronymDefinition
MGCKModified Group Cipher Key
References in periodicals archive ?
A qPCR master mix (2X) (1.5 [micro]L of reaction buffer 10X, 0.75 [micro]L of 50 mM MgCk, 0.3 [micro]L of 10 mM dNTPs, 0.75 [micro]L of EvaGreen[R] 20x [Biotium, Hayward, CA, USA]; 0.1 [micro]L of 5 U [micro][L.sup.-1] Biolase DNA Polymerase [Bioline, Taunton, MA, USA], and 4.1 [micro]L of ultrapure water) was prepared for all reactions of the experiment, separated in aliquots (reactions per plate), and stored at -20[degrees]C until use.
La reaccion de PCR se llevo a cabo en las condiciones siguientes: 1,5 mM MgCk, 1X del tampon comercial Taq ADN polimerasa (50 mM Tris-HCl pH 9,0 a 25 [grados]C, 50 mM KCL, 50 mM NaCl), 0,2 mM de cada dNTP, 1 [my]M de cada oligonucleotido (bar 5':5'-CGAGACAAGCACGGTCAACTTC-3' y bar 3':5'-GAA ACCCACGTCATGCCAGTTC-3') y 1 U de Taq ADN polimerasa (Fermentas, Alemania) en un volumen final de 25 En cada reaccion se usaron 100 ng de ADN genomico.
La mezcla de reaccion para el PCR consistio de amortiguador PCR 1X (10 mM TrisHCl, pH 8,5, 50 mM KCl), 1,5 mM MgCk, 0,2 mM dNTP's, 0,01 mM de cada iniciador y 1,5 U de Taq ADN polimerasa (Bioline, Boston MA, USA).