MHC IIMajor Histocompatibility Complex Class II
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Though immature dendritic cells synthesize large amounts of MHC II molecules, such MHC II molecules form [alpha][beta]-dimers that exhibit intracellular distributions.
No statistical significance difference was observed between the two methods used to generate BMDC MHC II molecules (Table 1).
Briefly, for cell surface staining, single splenic lymphocyte suspensions were washed twice and stained with anti-CD3, NK1.1, TLR2, TLR4, CD8, CD4, [gamma][delta]T, CD19, Ly6G, F4/80, MHC II, CD69, CD94 (NKG2A/C/E), or CD314 (NKG2D) antibodies for 30 min at 4[degrees]C in the dark.
These modifications precisely regulate CIITA's location, function, and stability within the cell and increase CIITA activity at the MHC II promoter [8, 10, 13-15, 19-22].
There was no obvious change in MHC II expression in DC cells from [TLR2.sup.-/-] mice immunized with MBP, BCG, or the combination of MBP and BCG (Figure 8(i)).
As shown in Figure 5, when MSCs were added for coculture 2h after DCs were plated (with the MSC:DC ratios of 1:1 and 1:10), the expression of CD80, CD83, CD86, and MHC II on the surface of DCs was significantly lower than that in DCs of DC only group detected by flow cytometry.
[sz]2M is believed to arise in a basal jawed vertebrate (gnathostome).[sup][8] The close proximity of MHC I, MHC II, and [sz]2M implies that they were derived from a common ancestor by tandem ( cis ) duplication.[sup][8] [sz]2M protein sequences are highly conserved among species, and overall structures are virtually identical.
Since the specific interactions between T cells and macrophages have been measured in a short (5 to 15 minutes) time period [23], we used an average value of 10 minutes to calculate the MHC II antigen presentation rate, giving a rate of 6 [h.sup.-1].
AGAF (100 [micro]g/ml) treatment induced DC maturation markedly by upregulating costimulatory molecules, whereas TLR2 and TLR4 siRNA transfection inhibited DC maturation (CD86: 53.1 [+ or -] 3.4% and 47.3 [+ or -] 7.4%, CD80: 72 [+ or -] 4.7% and 62.7 [+ or -] 5.4%, CD83:47.3 [+ or -] 9.8% and 56.3 [+ or -] 1.7%, CD40: 53.6 [+ or -] 4.9% and 49.3 [+ or -] 1.6%, MHC I: 71.2 [+ or -] 9.6% and 64.9 [+ or -] 7.6%, and MHC II: 62.8 [+ or -] 10.7% and 52.8 [+ or -] 11.9%, respectively) compared to AGAF treatment only (Fig.
Each helper T cell, derived from cells produced early in the body's development by the above-mentioned differentiation mechanism, has T-cell receptors on its surface that recognize a specific antigen attached to MHC II of the presenting cells.