For 21 cases, we processed entire MLBC material from the liquid fixative for cell blocks preparation without preparing LBC smears, to obtain adequate material for cell blocks (Table 1).
Of these, only 3 cases were reported as satisfactory on conventional smears and 7 cases were unsatisfactory on conventional smears and had inadequate samples for MLBC smear.
1 case of dysplasia was diagnosed as inflammatory on CPS and MLBC and other case of dysplasia was diagnosed as HSIL on CPS and MLBC.
This shows that cellular architecture is better preserved and neoplastic lesions which are underdiagnosed on CPS and MLBC can be correctly diagnosed on cell blocks.
MLBC is a cost-effective method which needs no expensive machine, uses only centrifuge and provides an improved slide preparation technique.
Material for MLBC was collected using plastic spatulas and simultaneous cervical scrape smears were made and stained with Pap stain.
In our study, we found that sensitivity of MLBC vs.
The possible explanations for improved sensitivity of MLBC may be improved sample collection, reduction in obscuring elements and better preservation of abnormal cells on the slide allowing for more definitive categorisation of abnormal cells as intraepithelial lesions.
In few of our cases, we entirely processed the material of MLBC without splitting the specimen, which yielded better results.
The widespread use of MLBC enables proper screening of tissue fragments obtained from cell block preparations.
The cell blocks prepared from MLBC specimens increase the diagnostic accuracy of Pap smears.