The expression levels of MLCK and PKC of the thoracic aorta were detected by the immunohistochemical method.
As shown in Figures 1 and 4, compared with the control group, the expression of cAMP-1 and PKA was decreased in all diabetic groups, whereas the expression of MLCK and PKC was increased (P < 0.
As shown in Figures 5 and 6, compared with the T2DM nonintervening group, the expression of MLCK and PKC was decreased in DDD-intervening groups (P < 0.
This study investigated the effect of early intervention with DDD on the expression of MLCK, cAMP-1, PKC, and PKA and gene expression in vascular endothelial cells of aortas of diabetic rats, to explore the roles of DDD on the permeability of vascular endothelial cells and its relationship with the MLCK signaling pathway.
MLCK plays an important role in modulating endothelial cell permeability.
Further mechanisms of investigation indicated that the effect of DDD intervention is related to upregulating the expression of cAMP-1 and PKA and downregulating the expression level of MLCK and PKC.
2+]/calmod-ulin-dependent protein kinase (CaMK); (ii) CaMK stimulates NOS to synthesize NO, which activates GC-S, leading to cGMP synthesis and release into the cytosol; (iii) cGMP activates PKG, which regulates myosin molecular motor activity, leading to granule translocation along the actin filaments constituting the cytoskeleton; (iv) myosin activity is determined by the degree of MLC phosphorylation/de-phosphorylation, which is controlled by the regulatory proteins MLCK, ROCK, and MLCP; and (v) RPCH uncoupling from the receptor leads to PDES-mediated reduction in cGMP concentration and PKG deactivation.
2+]/CaM complex, NOS, GC-S, PKG, PDE5, MLCK, ROCK, and MLCP in the intracellular signaling cascade consequent to RPCH binding to its membrane receptor in the red ovarian chromatophores of the freshwater shrimp Macrobrachium olfersi.
vi) Myosin light chain kinase, MLCK (phosphorylates MLC) [inhibitor ML-7 (1-(5-Iodonaphthalene-1-sulfony1)-1H-hexahydro-1,4-diazepine hydrochloride), 10 [micro]mol [L.
MLCK inhibition apparently has little effect on pigment movements and may not play a role in MLC phosphorylation.
myosin light chain; MLCP, MLC phosphatase; MLCK, MLC kinase; PKG, protein kinase NO, nitric oxide; NOS.