MLCLMediastinal Large B-Cell Lymphoma (hematology)
MLCLMille Lacs Community Library (Isle, MN)
MLCLMedium and Large Cell Lymphomas (dermatology)
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We found that LCA decreases the relative concentrations of the nonbilayer forming classes of phospholipids, which include the following: (1) PC, PI, PS, and PG phospholipids carrying only saturated acyl chains; (2) PE phospholipids with one or two unsaturated acyl chains; and (3) PA, CL, and MLCL phospholipids carrying either only saturated acyl chains or from one to four unsaturated acyl chains [50].
CDPDAG: cytidine diphosphate-diacylglycerol; CL: cardiolipin; DAG: diacylglycerol; ER: endoplasmic reticulum; IMM: inner mitochondrial membrane; IMS: intermembrane space; MAM: mitochondria-associated membrane domain of the ER; MICOS: mitochondrial contact site protein complex; MLCL: monolysocardiolipin; OMM: outer mitochondrial membrane; PA: phosphatidic acid; PC: phosphatidylcholine; PG: phosphatidylglycerol; PGP: phosphatidylglycerol-phosphate; PI: phosphatidylinositol; PS: phosphatidylserine.
Based on our experience with the analysis of MLCL/CL profiles in fibroblasts, lymphocytes, and other tissues, we found that bloodspots contain detectable amounts of MLCL and CL, which are sufficient to distinguish a BTHS patient from a healthy individual.
As a parameter to distinguish between BTHS and controls, we used the ratio between (a) the chromatographic peak area for the multiple reaction monitoring (MRM) of m/z 582.9 [right arrow] m/z 281.3 plus m/z 582.9 [right arrow] m/z 255.2 as a representation of the abundance of MLCL in the bloodspot and (b) the chromatographic peak area for the MRM of m/z 724.0 [right arrow] m/z 279.2 as a representation of the abundance of CL in the bloodspot.
By combining different amounts of control and BTHS bloodspots, we increased the MLCL content and concomitantly decreased the CL content.
Based on our previously developed methods to analyze phospholipids and specifically CL and MLCL in several tissues and types of cells, the HPLC elution profile was adjusted for CL and MLCL determination in bloodspot extracts.
The instrument variation for CL and MLCL in a control bloodspot extract was 13% and 44%, respectively.
A repeated extraction step increased the extraction yield by approximately 15% for CL and 25% for MLCL, which indicated that recovery from a bloodspot with the procedure is <85% for CL and <75% for MLCL.
Because the majority of material in dried bloodspots originates from erythrocytes, which do not contain mitochondria, the expected abundances for the biochemical markers of BTHS, CL and MLCL, were very low.
Therefore the actual value of this ratio is not the molar ratio of the MLCL and CL species in the bloodspot sample; it is a practical parameter that expresses the combined effect of two separate relevant indicators.