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mLDL was prepared by diluting low-density lipoprotein (Calbiochem; La Jolla, CA, USA) in phosphate buffered saline (PBS) and incubating with 10 [micro]M Cu[Cl.sub.2] at 37[degrees]C for 24 h [29, 30].
Cells were exposed to either 100 [micro]g/ml AGE or 500 [micro]g/ml mLDL with or without 100 [micro]M UDCA dissolved in DMSO.
Briefly, human retinal pericytes were plated on 24-well plates and exposed to either 100 [micro]g/ml AGE for 24 h or 500 [micro]g/ml mLDL for 12 h in 500 [micro]l medium, followed by 1 [micro]l of 1 [micro]g/ml PI dissolved in distilled water for 5 min at 37[degrees]C.
EM:51 Helga Franke G, N:149 MLDL Estudio Heemann U, Kohnle M, H:92 BSI observacional Luetkes P, Mae-hner M:57 K-22 descriptivo.
The rating continues to derive strength from MLDL's favourable capital structure, significant cash balance and liquid investments, the strong parentage of Mahindra & Mahindra Limited (rated LAA+(stable)/A1+ by ICRA) and MLDL's established track record in the real estate markets of Mumbai, National Capital Region (NCR), Chennai and Pune.
The positives are partly offset by the nascent stage of development of several industrial parks/SEZ projects being executed by MLDL's subsidiary companies that may warrant funding support from MLDL and uncertainty over the continuation of tax benefits for new units operating out of such SEZs.
Incorporated in December 2001, MLDL is the real estate and infrastructure development subsidiary of Mahindra & Mahindra Limited (M&M).
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