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The nucleotide sequences of the two exons of MLNR in the parent individuals were determined by polymerase chain reaction (PCR) amplification followed by direct sequencing to determine nucleotide variance of the gene in the resource family.
The ld_fix option of the Qxpak software (Perez-Enciso and Misztal, 2004) was used for the MLNR haplotypes.
On sequencing of the MLNR gene, we found GGGGC insertion polymorphisms located 54 bp upstream of the 5'-untranslated region and the sequenced region differed by 6 single nucleotide polymorphisms (SNPs) from the corresponding region in the draft sequence of the chicken genome.
We observed an association between three MLNR haplotypes (Haplotype 1, 2, and 3) and growth traits (Table 4).
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- MLN 19
- MLN 4
- MLN 51
- MLN 62
- MLN 70
- MLO projection
- MLO view
- Mloda Polska
- Mlodziezowa Rada Miasta Koszalina
- Mlodziezowy Ruch Rycerstwa Niepokalanej