MMEjMicrohomology-Mediated End Joining
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Other than HDR and NHEJ, the two major DNA repair pathways, recent studies have examined the potentials of the MMEJ repair pathway in mediating targeted knock-in of large DNAs.
On the other hand, recent studies have reported comparisons among the different knock-in approaches mediated by HDR, NHEJ, and MMEJ repair pathways, in cultured mouse ESCs as well as primary astrocytes and neurons [55].
They cut out a methylated promoter using CRISPR/Cas9 and then inserted an unmethylated one with MMEJ, substituting an on-switch.