MMLVMoloney Murine Leukemia Virus (also seen as MoMLV)
MMLVMontana Myotis Leukoencephalitis Virus
MMLVMouse Moloney Leukemia Virus
MMLVMoloney Murine Leukemia Virus-Derived (biochemistry)
MMLVMoms of Multiples of the Lehigh Valley (Pennsylvania)
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5 [micro]g RNA, 8 [micro]l 5x MMLV reaction buffer (Promega, Mannheim, Germany), 6 [micro]l dNTP's (300 [micro]M; Fermentas, St.
Reverse transcriptase reactions were subsequently performed before the PCR and in parallel with reactions without MMLV reverse transcriptase.
1M, 16 [micro]L de dNTPs, 1 [micro]L de ARN sin y 1 [micro]L de MMLV.
For mRNA reverse transcription reaction, 5 ug total RNA of each sample was performed with MMLV Reverse Transcriptase (Clontech, USA) according to the kit's manual.
Total RNA was isolated from murine liver tissue (strain C57BL/6) using trizol reagent (Invitrogen[TM]; ThermoFisher Scientific) and cDNA was synthetized using MMLV reverse transcriptase (Roche Diagnostics USA).
2 [micro]g of total RNA was used to synthesize the first strand cDNA by using OligodT primers and MMLV reverse transcriptase (Promega, Madison, WI, USA) according to the manufacturer's instructions.
The structural casting acts as chassis reinforcement for the front structure and adds stiffness, torque load capacity and torsional rigidity to the MMLV body structure.
RT was performed by using MMLV (Moloney murine leukemia virus) RT kit (Invitrogen, Carlsbad, CA, USA) and reverse primer 827R (nt 1777-1800) (Table) according to manufacturer's recommendations.