In the MMLV
, steel was placed in areas where requirements for energy absorption for side impact, roof crushing and frontal impact protection were high.
0 [micro]L (2 [micro]M) HT11M oligonucleotide primers and 1 [micro]L MMLV
5 [micro]g RNA, 8 [micro]l 5x MMLV
reaction buffer (Promega, Mannheim, Germany), 6 [micro]l dNTP's (300 [micro]M; Fermentas, St.
Reverse transcriptase reactions were subsequently performed before the PCR and in parallel with reactions without MMLV
1M, 16 [micro]L de dNTPs, 1 [micro]L de ARN sin y 1 [micro]L de MMLV
5 tag total RNA and 100 U MMLV
For mRNA reverse transcription reaction, 5 ug total RNA of each sample was performed with MMLV
Reverse Transcriptase (Clontech, USA) according to the kit's manual.
Total RNA was isolated from murine liver tissue (strain C57BL/6) using trizol reagent (Invitrogen[TM]; ThermoFisher Scientific) and cDNA was synthetized using MMLV
reverse transcriptase (Roche Diagnostics USA).
2 [micro]g of total RNA was used to synthesize the first strand cDNA by using OligodT primers and MMLV
reverse transcriptase (Promega, Madison, WI, USA) according to the manufacturer's instructions.
The structural casting acts as chassis reinforcement for the front structure and adds stiffness, torque load capacity and torsional rigidity to the MMLV
micro]g of RNA, 1U of MMLV
reverse transcriptase and random hexamer were used to synthesise cDNA.
RT was performed by using MMLV
(Moloney murine leukemia virus) RT kit (Invitrogen, Carlsbad, CA, USA) and reverse primer 827R (nt 1777-1800) (Table) according to manufacturer's recommendations.