MNSO

AcronymDefinition
MNSOGerman Minority of Lower Silesia (Poland)
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2 Supplied the following per kilogram of feed: Cu (CuSO 4 *5H 2 O) 3.5 mg; Fe (FeSO 4 *H 2 O) 50 mg; Zn (ZnSO 4 *H 2 O) 50mg; Mn (MnSO 4 *H 2 O) 2mg; I (KI) 0.14 mg; Se (NaSeO 3 ) 0.15 mg drip loss.
Fermentation technique: Twenty five milliliters of the fermentation medium consisting of (%, w/v); (NH 4 ) 2 SO 4, 0.14: KH 2 PO 4, 0.20; Urea, 0.03; MgSO 4, .7H 2 0, 0.03; ZnSO 4 .7H 2 O, 0.00014; FeSO 4 .7H 2 O, 0.0005; MnSO 4 0.00016; CoCl 2 , 0.0002; CaCl 2 , 0.0002; Tween-80, 2.0 ml; Polypeptone, 0.10; and sugarcane bagasse, 1 (pH 6.0) was transferred to the individual 250 ml cotton wool plugged conical flasks and autoclaved at 15 lb/inch 2 for 15 min.
Composition of the medium: A synthetic medium containing L-Glutamic acid, 20; L-Alanine, 0.2; Citric acid, 1; NaH 2 PO 4 .H2O, 2; KCl, 0.5; Na 2 SO 4 , 0.5; MgCl 2 .6H 2 O, 0.2; CaCl 2 .2H 2 O, 0.01; FeSO 4 .7H 2 O, 0.01; MnSO 4 .H 2 O, 0.01 was used in this study (Haavik, 1974).