To differentiate monocytes into MoDC
, cells were supplemented with recombinant porcine (rp) granulocyte-macrophage colony-stimulating factor (GM-CSF, 20 ng/ml) and rp IL-4 (50ng/ml; both from R&D Systems, Minneapolis, MN, USA).
At the time of data collection, LARS had 25 muskoxen and MODC
The hallmark IL-4 moDC
C-C chemokine receptor type 7 (CCR7) is equally high expressed on IL-15 EP DC and IL15/IL-15R[alpha] EP DC (Figure 1).
in Iselin, the MODC
plays an important role in the region's positive business climate.
The first relative clause center-embeds (MODC
), since it occurs to the left of the predicate, whereas the second one right-embeds (MODR), since it occurs to the right of the predicate.
Amongst these, ORF10 is of particular importance because its gene product is similar to the molybdate transporter protein ModC
. ModE is a molybdate-dependent regulator of several operons including the molybdate transport system of modABC.
[4.] Nonstandard abbreviations: RT-PCR, reverse transcription-PCR; PSA, prostate-specific antigen; QRT-PCR, quantitative RT-PCR; IS, internal standard; modC
, diaminohexanedeoxycytidine; nt, nucleotide(s); KLK1, tissue kallikrein; and KLK2, human glandular kallikrein 2.
The wild-type [CTA TAT TCA TCA TAG GAA ACA CCA AAG ATG ATA TT(modC
)5] and mutation-specific [CTA TAT TCA TCA TAG GAA ACA CCA ATG ATA TT[(modC
).sub.5]] targets and the allele-specific detection probes [wild-type = [(modC
).sub.20] ATC ATC TTT GGT, mutation = [(modC
).sub.20] TAT CAT TGG TGT] were obtained by conventional phosphoramidite synthesis on commercial supports.
Monocytes and moDC
were stained for HLA-DR (clone B8.11.2, IgG2b), HLA-A2 and CD14 (both from BD Biosciences), CD206/"mannose receptor" (MR, clone D547.3, IgG1 ), CD209/DC-SIGN (R&D, IgG2a), and CD89 (clone 2D11, IgG1 ).
Diaminohexane-modified deoxycytidine phosphoramidite (modC
), active lanthanide chelates, streptavidin-coated microtitration plates, DELFIA Assay Buffer, Wash Solution, and Enhancement Solution, and a DELFIA Plateshake, Platewash, Plate Dispense, and 1234 Research Fluorometer were from Wallac Oy (Turku, Finland).
Generation of Monocyte-Derived Dendritic Cells (moDC
Nonclassical DCs can be further divided into three major subsets: plasmacytoid DCs (pDCs), monocyte-derived DCs (moDCs
), and Langerhans cells (LCs) .