UGT1A9 and UGT2B7 are the principal enzymes responsible for MPAG
The platelet aggregation curve was recorded for 5 min with MPAG
as the analyzed parameter.
In present study, blood Pressure profile was not found to be significantly different between girls of high physical activity group (HPAG), moderate physical activity group (MPAG
) and low physical activity group (LPAG) despite a significantly lower waist circumference (WC) in HPAG girls as compared to girls of LPAG (Table 1).
, AcMPAG, and the carboxybutoxy ether of MPA (MPAC) were kind gifts from Roche Pharmaceuticals.
Several HPLC-tandem mass spectrometry (MS/MS) methods for monitoring MPA, free MPA, and MPAG
in serum or plasma have been reported.
Although the precise mechanism leading to the observed losses is unknown, we recommend use of polystyrene tubes or methanol as solvent for MPA and MPAG
calibrators and stock solutions.
MPA is metabolized mainly to a glucuronide derivative (MPAG
), which is believed inactive; however, the MPAG
concentrations in sera of patients treated with MMF are an order of magnitude higher than those of MPA, and thus its potential for interference in the various analytical methods is high.
The remaining samples had to be injected either at a reduced volume because of MPAG
concentrations exceeding the capacity of the UV detector or at a higher volume because of low AcMPAG concentrations.
The phenolic glucuronide of mycophenolic acid, (MPAG
), the primary metabolite of MPA, is produced by UDP-glucuronosyltransferase(s) in liver and possibly other tissues such as kidney and small intestine.
Most studies on the pharmacokinetics of MPA have utilized HPLC procedures (6, 7) to measure both MPA and MPAG
. Recently, the first immunoassay became available for the quantification of MPA (Emit-MPA, Dade Behring).
MPA is converted in the liver mainly to the 7-O-glucuronide metabolite, mycophenolic acid glucuronide (MPAG
), which does not have immunosuppressant activity (3, 5).
To address these questions, accurate and sensitive methods are needed to determine the free and total MPA concentrations, as well as the concentration of its glucuronide metabolite (MPAG
) in patient blood specimens.