Master mix was prepared using AccuPower[R] Multiplex PCR PreMix kit (Bioneer Company, South Korea).
Therefore, the increase in group size is counterbalanced by the decrease in the MPCR
. Moreover, the standard game-theoretic equilibrium and the social optimal choices remain unchanged (see Section III for details).
Multiplex PCR (mPCR
) for the detection of Salmonella spp.
Recently, nested mPCR
(nmPCR) (16-18) and 2-stage amplification combining PCR and loopmediated isothermal amplification (LAMP) (isoPCR) (19, 20) have emerged as powerful methods to concurrently detect multiple pathogens.
For the mPCR
specificity evaluation, these samples were artificially and separately inoculated with a loopful of culture from Baird-Paker Agar (strains identified as CPS) incubated at 37[degrees]C for 48h.
There are also a number of commercialized mPCR
tests which include the detection of M.
(262) For such groups, the increase in size decreases MPCR
, thus decreasing cooperation.
Matrizes Progressivas Coloridas de Raven (MPCR
; Angelini et al., 1999).
= 0.75 was used in all treatments for tokens contributed to the group fund.
 The early testing protocol involved tracking the inheritance of the putative X chromosome using intragenic linkage markers and later included the deletion hotspot screen with multiplex polymerase chain reaction (mPCR
), based on the work published by Chamberlain et al.
Molecular characterization of strains was performed by conventional multiplex PCR (mPCR
) test, as previously published.
The compared algorithms are MPCR
 and CASNCP which will be described in the next subsection.