A commercially available Olympus FV1000MPE (Olympus America, Center Valley, Pennsylvania) multiphoton laser scanning microscope (
MPLSM or MPM) was used for this study.
The fabrication method is analogous to multiphoton laser scanning microscopy (
MPLSM) in that the excitation, and thus, the photochemistry is restricted to the focal volume [17].
One is called multiphoton laser-scanning microscopy (
MPLSM), which is an advanced fluorescence imaging technology that is now commercially available at the high end of the microscope market.