A human pancreatic adenocarcinoma Capan1 cell line, human cervix carcinoma HeLa cell line, human lung fibroblasts MRC5
cell line, human breast adenocarcinoma MCF7 and HCC1937 cell line were obtained from American Type Culture Collection (Bethesda), and maintained as exponentially growing monolayers by culturing according to the supplier's instructions in a humidifier incubator at 37[degrees]C supplied with 5% C[O.sub.2].
The slides were examined using an Axiophot Photomicroscope equipped with an AxioCam MRc5
. Each slide was evaluated according to the intensity of positive immunostaining.
For example, siRNA duplexes directed against the Rift Valley fever virus (RVFV) nucleoprotein can effectively inhibit RVFV replication in human (MRC5
cells) and African green monkey cells (Vero E6 cells).
The cytotoxic effect of this molecule toward HepG2 cells was particularly evident, whereas no significant toxic effect was observed in MRC5
or HFF-1 human fibroblasts.
The presence of [eGFP.sup.+] cells in them was revealed with an AxioCam Z1 fluorescence microscope equipped with an AxioCam MRc5
color digital camera (Carl Zeiss, Germany).
Inactivation of p16INK4a, with retention of pRB and p53/ p21cip1 function, in human MRC5
fibroblasts that overcome a telomere-independent crisis during immortalization.
The specimens were pictured with the digital camera AxioCam MRc5
(ZEISS, Japan) following the processing of pictures in Abode Photoshop Elements 11.
Human breast carcinoma cells (MCF7), human cervical carcinoma cells (Ca Ski), human lung carcinoma cells (A549), human colon carcinoma cells (HCT116 and HT29), and noncancerous human lung fibroblast cells (MRC5
) were purchased from the American Tissue Culture Collection (ATCC, USA).
Sections of 5 [micro]m were stained with 0.1% (w/w) Oil Red O, mounted in glycerol, and observed at an Axio Cam MRc5
microscope equipped with camera driven by AxioVision 4.6 software (Carl Zeiss, Germany).
Digital habitus photographs were taken using a high resolution microscopy camera AxioCam MRc5
mounted on a Zeiss Axio Zoom V16 microscope.
The images were obtained with a digital camera, AxioCam MRc5
(Carl Zeiss Group, Goettingen, Germany) attached to the microscope.