We use the modified resistant hepatocyte model (MRHM), in order to induce preneoplastic lesions in the liver of rats (FT group) and to evaluate the capacity of quercetin for reversing the lesions (FT + Q group).
As we expected, the induction of preneoplastic lesions by using the MRHM resulted in a 74% decrease of IGF-1 expression (Figure 4(d), FT).
The ability of quercetin to reverse preneoplastic lesions in the liver was tested in the MRHM. This model has been used by us  and other authors in order to observe the three different stages associated with the development of HCC: initiation, promotion, and progression [11, 16, 17].
HCC: Hepatocellular carcinoma MRHM: Modified resistant hepatocyte model DEN: N-Diethylnitrosamine 2-AAF: 2-Acetylaminofluorene GGT: Gamma-glutamyl transpeptidase EGFR: Epidermal growth factor receptor PI3K: Phosphoinositide 3-kinase MAP: Mitogen-activated protein NT: Nontreated group Jak1: Janus tyrosine kinase 1.