Few studies using MSDB that have explored the neurobiological and behavioral effects, had grouped or analyzed according to each sex group [16-18].
Taking in to account all these antecedents, the objective of the present study was to identify the possible changes induced by MSDB in the total cell counting of GFAP and non-GFAP cells, along different areas including motor cortices of the rat brain and to describe whether these changes were different between male and female groups, comparing all data with control groups.
The MSDB protocol was the same as reported in previous studies .
In fact, alterations of the axis that regulates the response to stress have been reported using MSDB model in rats .
Some articles have detailed the morphometrical changes in the gross anatomical thickness of these cortices , but using a protocol of maternal deprivation that is not fully related to the MSDB protocol used in this study.
It is important to note that these increases in GFAP-marked cell count were not observed in female subjects, indicating the presence of other factors affecting the GFAP-positive cells population, leading to a more complex needing for understanding the role of those cells in this MSDB protocol scenario, according to the subject's sex group.
Out of total trypsin-digested peptide fragments (Figure 2) of Nivulian II, only thirteen peptides that is, 28.50% sequence was hit in MSDB
(mass spectrometry protein sequencing database) by Mascot peptide fingerprint search engine with Maturase K (Q52ZV1_9MAGN) of Banksia quercifolia (Family: Proteaceae) with 67.1 score and P < 0.05 (Figure 3(a)).