These virulence genes are mainly associated with host cell recognition and invasion (invA, orgA, prgH, tolC, sopB, pefA), intracellular survival and growth in reticuloendothelial tissues (pagC, spvC), survival within macrophages (spiA, msgA
), entry into non-phagocytic cells and killing of macrophages (spaN, sipB), and iron acquisition (ironN) (MOHAMED et al., 2014; SKYBERG et al., 2006).
Amplification was performed in a thermocycler (MJ Research, PTC 200 USA) under the following conditions: 1 cycle of initial denaturation at 95[degrees]C for 5 min, denaturation at 94[degrees]C for 30 sec, and annealing for 1 min at 53[degrees]C for spvB, spiA, pagC, and msgA and at 56[degrees]C for sipB and spaN and 2 min at 72[degrees]C, with a final cycle of 10 min at 72[degrees]C, followed by a hold at 4[degrees]C.
As shown in Figures 3(a) and 3(b) and Table 4, majority of Salmonella isolates carried spiA, pagC, and msgA genes regardless of origin.
All the 11 isolates in serogroup E had sipB and spaN whereas 10 had msgA, pagC, and spiA.
IgG responses against MsgA, MsgB, MsgC1, MsgC3, MsgC8, and MsgC9 were measured.
All staff had detectable antibodies against all Msg fragments: MsgA, MsgB, MsgC1, MsgC3, MsgC8, and MsgC9 (Table 2).
Participants had detectable antibody levels to MsgA (GM 11.8, 95% CI 8.1-17.0), MsgB (GM 2.6, 95% confidence interval [CI] 2.1-3.1), and MsgC1 (GM 17.8, 95% CI 13.8-22.9) (Table 2).
Antibody levels to MsgA or MsgB did not differ significantly by age, sex, race, ethnicity, smoking status, presence of chronic lung disease, or presence of immunocompromising condition (Table 2).
In this pilot study, we sought to determine whether serum antibody levels to MsgA, MsgB, and MsgC differed in HIV-positive patients with acute pneumonia due to P.
Serum antibody levels to MsgA, MsgB, and MsgC were measured in a blinded manner by an ELISA as previously described (14,17,18).
A standard serum sample, obtained from a healthy donor with known reactivity to MsgA, MsgB, and MsgC in Western blot, is run on each plate as a control.
Variations in assay results using the control serum were measured for MsgA and MsgC on a per-plate basis (n = 6, coefficients of variation [CV] 3% 5%for MsgA and 3.6%-7% for MsgC), a daily basis using two plates (n = 12, CV 3.3%-5.8% for MsgA and 4.8% 7.4% for MsgC), and an overall basis (across 4 days with two plates per day) (n = 48, CV 8.7% for Msg A and 13.3% for MsgC).