We conclude that the IBU has been successfully loaded on the surface of the mesoporous silica, that NQ-62 is successfully grafted on the surface of the mesoporous silica, that the MDNQ is successfully grafted on the surface of MSN, and that the template agent is almost completely removed.
The MDNQ-modified MSN has an increased loading rate.
The amount of loaded ITZ into MSN after storage in the nanopatrticles was measured three times by UV-Spectrophotometric method at [lambda] = 261 nm (1201, Shimadzu Co.
Chemical structure of MSN, calcinated MSN, MSN-ITZ and MSN-ITZ-CHI were evaluated by FTIR method.
MSN, MSN-ITZ and MSN-ITZ-CHI synthesis and characterization
Figure 3 is shown XRD patterns of MSN (Fig.3.A) and MSN-ITZ-CHI (Fig.3.B).
The tumor accumulation could be further improved by attaching a targeting moiety to MSNs, the researchers said.
The treatment of mice with camptothecin-loaded MSNs led to shrinkage and regression of xenograft tumors.
By the end of the treatment, the mice were essentially tumor free, and acute and long-term toxicity of MSNs to the mice was negligible.
Next, 180 mg of FA-PEG was coupled to the ammoniated MSNs using EDC and NHS as activation agents at 25[degrees] C for 12 h.
Pure PTX, MSNs, physical mixtures of PTX and MSNs (PPM), physical mixtures of PTX and FA-PEG-MSNs (PPFA), MSNs-PTX, and FA-PEG-MSNs-PTX were monitored by DSC and XRD to characterize the solid state of PTX.
To obtain FITC fluorescence-labeled MSNs (FITC-MSNs) and FA-PEG-MSNs (FITC-FA-PEG-MSNs), FITC was dissolved in 1 ml of absolute alcohol and then 100 mg of ammoniated MSNs or FA-PEG-MSNs was added to the solution.