MSPCR

Acronym	Definition
MSPCR	Mutagenically Separated Polymerase Chain Reaction Assay
MSPCR	Mutation Specific Polymerase Chain Reaction

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The samples were scored as being indicative of AS, PWS, or unaffected, and the results were compared with the original diagnosis obtained by MSPCR. Once the assays had been fully optimized, the 152 samples were correctly identified; the results are shown in Fig.

Quantitative analysis of SRNPN gene methylation by pyrosequencing as a diagnostic test for Prader-Willi syndrome and Angelman syndrome

Nevertheless, as with any multiplex PCR, we recommend that users optimize the conditions, especially the primer concentrations for the MSPCR, in each laboratory.

Multiplexed mutagenically separated PCR: simultaneous single-tube detection of the factor V R506Q (G1691A), the prothrombin G20210A, and the methylenetetrahydrofolate reductase A223V (C677T) variants