Taking MSRV as an example, the input data for predicting a single amino acid substitution that results from a single base alternation in protein coding sequence includes the protein name, the amino acid substitution, and position of the substitution in protein sequence, and the output data includes the prediction score ranging from 0 to 1, where 0 stands for neutral nsSNP and 1 means deleterious nsSNP.
MSRV provides an easy and effective way to calculate the conservation scores for the original and substitute amino acid, which are the frequencies of occurrences of the amino acids in the corresponding position of the Pfam multiple sequence alignment.
MSRV adopts six physicochemical properties of amino acids, including molecular weight, pI value, hydrophobicity scale, and relative frequencies for the occurrences of amino acids in the secondary structures (helices, strands, and turns) of proteins with known secondary structural information.
Capable of detecting Salmonella species within 24 hours, Modified Semi-solid Rappaport Vassiliadis (MSRV) medium is one of a range of products from Lab M devoted to the detection and isolation of these organisms.
MSRV medium is inoculated directly from pre-enrichment culture to the centre of the plate.