MUCRP1Multi-Ubiquitin-Chain Reference Preparation 1
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The lipoprotein-free serum derived from 110 /,L of the serum was applied to the column and eluted with the same buffer used for the separation of MUCRP1. The MT of the fractionated proteins was estimated as described above.
Concentrations of multiubiquitin chains and free ubiquitin in a total of 30 serum samples from seven AVH patients were 9.62 [+ or -] 4.58 [micro]g/L (in terms of MUCRP1; range 3.51-19.2) and 133 [+ or -] 57.2 [micro]g/L (range 52.2-261), respectively.
(6) Nonstandard abbreviations: AVH, acute viral hepatitis; SLE, systemic lupus erythematosus; RA, rheumatoid arthritis; MND, motor neuron disease; MS, multiple sclerosis; MUCRP1, multiubiquitin chain reference preparation 1; AST, aspartate aminotransferase; ALT, alkaine aminotransferase; LDH, lactate dehydrogenase; ALP, alkaline phosphatase; LAP, leucine aminopeptidase; [gamma]-GTP, [gamma]-glutamyltranspepfidase; CRP, C-reactive protein; DBi, direct bilirubin.