The MALDI-8020 mass spectrometer is said to mark the company's return to benchtop matrix assisted laser desorption
ionization time of flight mass spectrometry (MALDI-TOF MS).
Microbiology, veterinary medicine, and other researchers from Europe and the US provide nine chapters on the application MALDI-TOF (matrix assisted laser desorption
ionization time-of-flight) mass spectrometry in various areas of microbiology--clinical and veterinary diagnostic laboratories, food microbiology, environmental microbiology, and strain collections--and impacts on laboratory practice and patient outcomes.
MALDI-MS (Matrix assisted laser desorption
ionization mass spectroscopy) refers to a rapid, versatile, in vitro technique for molecules identification based on photo-desorption of a sample embedded within a light absorbing matrix (9).
It is the general consensus among microbiology and infectious disease specialists that molecular detection technology, whether protein-based assays such as electrospray mass spectrometry (ESI-MS), MALDI-TOF (matrix assisted laser desorption
ionization time of flight), or DNA-based technologies, is the future of microbiology.
Then, to determine the products of the chemical reaction, they used a type of laser system known as MALDI (for Matrix Assisted Laser Desorption
and Ionization), which involves zapping the ice with both infrared and ultraviolet lasers.
ABREVIATIONS: 2-DE = two-dimensional electrophoresis; CHAPS = (3-[ (3-Cholamidopropyl)-Dimethylammonio]-1-Propane Sulfonate); ESI - MS = electrospray ionization mass spectrometry; ICAT = isotopecode affinity tag; IPG = immobilized pH gradient; LC-MS = liquid chromatography mass spectrometry; MALDI = matrix assisted laser desorption
ionization; MALDI-TOF = matrix assisted laser desorption
ionization time-of-flight; mRNA = messenger ribonucleic acid; pI = isoelectric point; SELDI-MS = surface-enhanced laser desorption/ionization time-of-flight.
Matrix assisted laser desorption
ionization/time-of-flight-mass spectrometry is then used to produce a mass spectrum that is analyzed to determine if one or more proteins associated with the bacteriophage are present, thereby indirectly indicating that target bacterium were present in the liquid mixture.
The system is an API (Atmospheric Pressure Ionization) hybrid IT (Ion Trap) and TOF (Time-of-Flight) MS, in contrast to the company's Axima QIT-TOF, which is a MALDI (Matrix Assisted Laser Desorption
Serum proteins are selectively bound to chemically active surfaces on biochips and rapidly scanned to obtain a spectrum of protein masses by a modified MALDI-Tof (matrix assisted laser desorption
ionization--time of flight) MS instrument.
Wesdemiotis, Structural characterization of quinoxaline homopolymers and quinoxaline/ether sulfone copolymers by matrix assisted Laser desorption
ionization mass spectrometry, Anal.