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," Archaea, vol.
Shotgun proteomics of Methanococcus jannaschii
and insights into methanogenesis.
flap endonuclease: expression, purification, and substrate requirements.
Schonheit, "Novel type of ADP-forming acetyl coenzyme A synthetase in hyperthermophilic Archaea: heterologous expression and characterization of isoenzymes from the sulfate reducer Archaeoglobus fulgidus and the methanogen Methanococcus jannaschii
" Journal of Bacteriology, vol.
bovis AF2122/97 SCO Bacteria Actinobacteria Streptomyces coelicolor A3(2) SMA Bacteria Actinobacteria Streptomyces avermitilis RBA Bacteria Planctomyces Rhodopirellula baltica BTH* Bacteria Bacteriod Bacteroides thetaiotaomicron CTE Bacteria Green sulfur Chlorobium tepidum TLS TMA Bacteria Hyperthermophilic Thermotoga maritime MSB8 MJA Archaea Euryarchaeota Methanococcus jannaschii
DSM2661 MAC Archaea Euryarchaeota Methanosarcina acetivorans C2A MMA Archaea Euryarchaeota Methanosarcina mazei Goel MTH Archaea Euryarchaeota Methanobacterium thermoautotrophicum deltaH MKA Archaea Euryarchaeota Methanopyrus kandleri AV19 AFU Archaea Euryarchaeota Archaeoglobus fulgidus VC-16 HAL Archaea Euryarchaeota Halobacterium sp.
Last summer, researchers sequenced the entire genome of Methanococcus jannaschii
, a methane-generating species of archaea living on the ocean floor (SN: 8/24/96, p.
Due to their stability and ease of purification from heterologous expression systems, the archaeal P.furiosus and Methanococcus jannaschii
Fen1 proteins were the first to be crystallized and structurally characterized [55, 98].
We purified, cloned, and expressed the enzyme from the thermophile Sulfolobus shibatae (11), and identified homologs in the published genomic sequences of Methanococcus jannaschii
(1), Methanobacter thermoautotrophicum (2), and Archaeoglobus fulgidus (3).
The single-celled microorganism Methanococcus jannaschii
made headlines last month when scientists announced that they had fully sequenced its genes (SN: 8/24/96, p.
Recently, this dogmatic view has been called into question by the sequences of a number of archaeal genomes; for example, the genomic sequence of Methanococcus jannaschii
does not contain open reading frames (ORFs) encoding homologs of the asparaginyl-, cysteinyl-, glutaminyl-, and lysyl-tRNA synthetases (1-3).
This week, scientists announced that they have finished sequencing all the genes of the deep-sea microbe, a crucial step in comprehending how the unusual microorganisms, known as Methanococcus jannaschii
, flourish without using sunlight, oxygen, or any surrounding organic material.