All pyrazinamide-resistant strains detected with the MGIT
960 system (n = 33, 71.7%) had a mutation in the pncA gene or its promoter.
Use of BACTEC MGIT
960 for recovery of mycobacteria from clinical specimens: Multicenter study.
For BACTEC[TM] MGIT
960[TM] TB system (also applicable for manual MGIT
) mycobacteria growth indicator tube (MGIT
) culture and drug susceptibility demonstration projects; 2006.
The chromatographic immunoassay was carried out as per manufacturer's instructions (BD MGIT
TBc Identification Test).
that demonstrated the presence of contaminants on staining and blood agar were subcultured on Lowenstein-Jensen slopes with antibiotic supplement and incubated for another 28 days at 37[degrees]C.
Furthermore, all the samples were cultured in Middlebrook 7H9 medium using Manual BBL MGIT
system as per the protocols  and were followed upto 6 weeks for Mycobacterial growth.
[25-27] Use of the GenoType MTBDRplus assay has been shown to reduce time to initiation of appropriate drug therapy by 25 days compared with BACTEC MGIT
960 in rural SA.
tuberculosis culture and drug susceptibility testing were performed for first-line drugs (isoniazid, rifampicin, ethambutol, and pyrazinamide) using the automated Mycobacterium Growth Indicator Tube (MGIT
960 system; BD Diagnostics, Sparks, MD, USA).
Rapid drug susceptibility methods like BACTEC and MGIT
should be implied on a larger scale.
In recent years, the BACTEC MGIT
960 system, a fully automated and nonradiometric culture system, has been recommended for faster mycobacterial isolation from clinical specimens .
Non-Beijing n = 16 Demographics Males, n (%) 14 (87.5%) Age [y, mean (%)] 50.7 Mycobacterial identification AFB-positive strains, n (%) 13 (81.2%) Time to growth in MGIT
(weeks) 743 Drug resistance profile Isoniazid-resistant strain 0 MDR strain 2 (12.5%) Laboratory findings CRP (mg/mL) 6.55 [+ or -] 4.38 Blood IgG (mg/mL) 1767 Blood IgA (mg/mL) 3.83 Chest radiograph findings Cavity, positive (%) 4 (26.7%) Pleural effusion, positive (%) 3 (20.0%) Beijing n = 20 p value Demographics Males, n (%) 14 (70.0%) n.s.
Subsequently, the mycobacterial species was determined by mycobacterial growth indicator tube (MGIT
) culture to be Mycobacterium marinum in two additional joint aspirates (left wrist and olecranon bursa) that were grown at reduced temperature.