MGB Biopharma's anti-infectives are based on its minor groove binder
Addition of a minor groove binder
(MGB) to the probe sequence at the 3' end of the probe permits it to anneal at a higher melting temperature (Tm), allowing the use of shorter more specific probes (2,18).
DAPI is an AT-selective minor groove binder
. Binding to dsDNA produces a ~20-fold fluorescence enhancement, emits blue fluorescence upon excitation with mercury-arc lamp or UV lines of the argon-ion laser (Ex/Em = 358/461 nm).
The minor groove binder
(MGB) probe technology overcomes some of these obstacles.
This assay amplifies and detects a conserved region upstream of the Rotbart amplicon by using primers containing degenerate and modified bases and a minor groove binder
(MGB)-conjugated hybridization probe.
(PERK) in oysters, using TaqMan[R] assays designed with Minor Groove Binder
(MGB) probes on an Applied Biosystems 7500 Real-Time PCR System.
The compound is a DNA minor groove binder
and is currently in pre-clinical development with IND filing scheduled for the third quarter of this year.
Recently, a real-time PCR assay based on minor groove binder
(MGB) probe technology was developed for rapid identification and characterization of the antigenic variants.
 Nonstandard abbreviations: SNP, single-nucleotide polymorphism; MGB, minor groove binder
; RAPID, Ruggedized Advanced Pathogen Identification Device; USAMRIID, United States Army Medical Research Institute of Infectious Disease.
Recently, minor groove binder
(MGB) probe assays have been established for characterization of CPV strains and account for the presence of single nucleotide polymorphisms in the genome of the different variants (5).
Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder
. Nucleic Acids Res 1997;25:2657-60.
Quantitation of severe acute respiratory syndrome coronavirus genome by real-time polymerase chain reaction assay using minor groove binder
DNA probe technology.