DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I
from diverse metazoan invertebrates.
Mitochondrial cytochrome c oxidase subunit I
(COX-I) is the central catalytic subunit of cytochrome c oxidase (complex IV), and COX-I gene is used as a standard marker for DNA bar coding to enable species identification in animals [3, 13].
Ham et al., "Molecular identification of necrophagous muscidae and sarcophagidae fly species collected in Korea by mitochondrial cytochrome C oxidase subunit I
nucleotide sequences," The Scientific World Journal, vol.
Nucleotide sequences, in particular standardized genomic regions, such as those of the mitochondrial cytochrome c oxidase subunit I
(COI) and cytochrome b (Cyt b) genes, have been proposed as important tools for the identification of species.
Methods: Phylogenetic inference calculations were based on partial mitochondrial cytochrome c oxidase subunit I
(COX 1), 16S ribosomal RNA and nuclear histone subunit 3 (H3) genes using maximum likelihood, maximum parsimony and Bayesian approaches.