3] Nonstandard abbreviations: RPA, recombinase polymerase amplification; POC, point-ofcare; SSB, single-strand DNA binding protein; dsDNA, double-stranded DNA; RT-RPA, reverse transcriptase RPA; THF, tetrahydrofuran; dR, deoxyribose; MuLV
, murine leukemia virus; LF, lateral flow; MRSA, methicillin-resistant Staphylococcus aureus; LAMP, loop-mediated amplification.
v] = voltage-gated potassium channel, MBP = myelin basic protein, MHC II = major histocompatibility class II, MS = multiple sclerosis, MuLV
= murine leukemia virus, NADPH = nicotinamide adenosine dinucleotide phosphate, NO = nitric oxide, NOS = NO synthase, PNS = peripheral nervous system, T cells = T lymphocytes.
Total RNA from each sample was reverse transcribed with MuLV
reverse transcriptase and Oligo d(T) primers (Applied Biosystems, Foster City, CA).
In the southwestern United States, LSCV, SNV, ELMCV, and MULV
are principally associated with rodents of the species P.
was submitted to a first round reverse transcription with MuLV
enzyme, followed by a hot start PCR with the AmpliTaq[R] Gold.
According to the protocol, total RNA was incubated for 5 min at 65[degrees]C at first because the desired genes were rich in %G+C and then 60 min incubation with MuLV
reverse transcriptase at 42[degrees]C was done.
Total RNA was collected using TRIzol reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy Mini Kit columns (Qiagen, Valencia, CA) according to the manufacturer's protocol, and transcribed with MuLV
(Moloney murine leukemia virus) reverse transcriptase and oligo-dT primers.
Purified total RNA was reverse-transcribed with MuLV
reverse transcriptase and oligo-dT primers.
Reverse transcription was performed using 500 ng of total RNA in the presence of random examers and MuLv
reverse transcriptase (Applera Corporation, USA).
Then 3 [micro]g RNA was reversed transcribed into cDNA in a final volume of 20 [micro]l containing 100 pmol random hexamer, and 50 U of MuLV
reverse transcriptase (New England Biolab) according to the manufacturer's guideline.
Purified RNA was reverse transcribed with MuLV
(murine leukemia virus) reverse transcriptase and oligo-dT primers.