MuLVMurine Leukemia Virus
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were the first to evaluate different RT enzymes with RPA reagents and demonstrated that Transcriptor RT performs better than MuLV RT (10 molecules detected with Transcriptor RT vs 102 molecules with MuLV RT) and Sensiscript RT (Qiagen; 103 molecules detected) when combined to exo kit in an assay for detecting the Rift Valley fever virus (24).
In another model of neuroinflammatory disease, PVC-211 murine leukemia virus (MuLV)-induced spongiform neurodegenerative disease in rats, a highly reactive microglial/ macrophage response is associated with severe free-radical injury, motor neuron injury, and death.
Featuring a larger, 50% brighter screen and exceptionally long battery life, the 3.3 pound LifeBook B6000 notebook, powered by the Intel Pentium MULV 753 process or with enhanced SpeedStep technology delivers the flexibility of a touch screen and the productivity of a notebook for maximum efficiency.
Reverse transcription and first PCR were performed in 100 [micro]L of mixture containing 50 mL of HCV RNA suspension, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2 mM MgCl2, 0.2 mM dATP, 0.2 mM dGTP, 0.2 mM dTTP, 0.2 mM dCTP, 8 U of RNase inhibitor, 50 [micro]M MuLV reverse transcriptase, 2.5 U of Taq polymerase, 0.4 [micro]M outer primer pairs (sense, 5'-GCGACACTCCACCATAGAT-3', 2 to 20, and antisense, 5'-GCTCATGGTGCACGGTCTA-3', 312 to 330).
Discrete regions of sequence homology between cloned rodent VL30 genetic elements and AKV-related MuLV provirus genome.
MuLV reverse transcriptase was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Then, we added MuLV reverse-transcriptase (2 U) and incubated the mixture at 37[degrees]C for 60 min and 70[degrees]C for 10 minutes.
Briefly, 1 pg total RNA was reverse-transcribed by MuLV reverse transcriptase in the presence of random hexamers followed by the indicated cycles of PCR (95[degrees]C for 1min, 55[degrees]C for 1min, and 72[degrees]C for 1 min) in the presence of 2 [micro]M VEGF-specific primers (28 cycles) or [beta]-actin-specific primers (16 cycles) as a control.
Total RNA from each sample was reverse transcribed with MuLV reverse transcriptase and Oligo d(T) primers (Applied Biosystems, Foster City, CA).
Other hantaviruses that are principally associated with neotomine or North American sigmodontine rodents include Carrizal virus (CARV), Catacamas virus, El Moro Canyon virus (ELMCV), Huitzilac virus (HUIV), Limestone Canyon virus (LSCV), Montano virus (MTNV), Muleshoe virus (MULV), Playa de Oro virus, and Rio Segundo virus (RIOSV) (8-14).
RNA Isolation, Amplification and Genotyping Plasma viral RNA isolated and purified by ultra centrifugation (120,000g for 120 min.) was submitted to a first round reverse transcription with MuLV enzyme, followed by a hot start PCR with the AmpliTaq[R] Gold.