aureus and MRSA cultured on Mueller Hinton broth were incubated at 37[+ or -][degrees]C for overnight.
Wells were filled with 20 [micro]l of overnight culture (1x106 CFU/ml) and 180 [micro]l of Mueller Hinton broth followed by 100 [micro]l of different concentrations of highly active fractions.
The assays were set by preparing serial dilutions containing Cu NPs at concentrations of 200, 400, 800, 1600, and 3200 [micro]g [mL.sup.-1], which were suspended in Mueller Hinton broth
1 mL Mueller hinton broth dilution was added to each test tube.
Diluted solutions were added to the sterilized Mueller Hinton broth dilutions.
baumannii isolate was prepared by harvesting cells from 16-24 hour growth on the TSA II plate and suspending the cells in cation adjusted Mueller Hinton Broth
(MHB) (BBL/BD, Sparks, MD) and adjusting the turbidity to equal a 0.5 McFarland turbidity standard (approximately 1.5 x 10 (8) cells/mL).
The bacterial strains preserved on nutrient agar at 4[degrees]C were revived in Mueller Hinton broth
and incubated overnight at 37[degrees]C.
Antimicrobial-drug susceptibilities were determined by microbroth dilution by using Sensititre STP3F panels (Nova Century Scientific Inc., Burlington, Ontario, Canada) and cation-adjusted Mueller Hinton broth
with lysed horse blood (2%-5% vol/vol) by TREK Diagnostic Systems, Inc.
Minimum inhibitory concentrations (MICs) were performed by the microbroth dilution method parallel in Mueller Hinton broth
and glucose added urine.
Mueller Hinton Broth
(Oxoid Hampshire England)) tubes were inoculated with different bacterial cultures.
penicillin G/ml Mueller Hinton broth Mueller Hinton broth
The isolates were propagated on autoclaved Mueller Hinton broths
(HiMedia, India) for 18 hrs.