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MPCRMultiplex Polymerase Chain Reaction
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References in periodicals archive ?
Najera-Sanchez G, Maldonado-Rodriguez R, Olvera PR, Garza LM (2003) Development of two multiplex polymerase chain reactions for the detection of enterotoxigenic strains of Staphylococcus aureus isolated from foods.
Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction. FEMS Microbiol Lett.
Prevalence of high risk genital papillomaviruses in the Belgian female population determined by fast multiplex polymerase chain reaction. J Med Virol 1992;36:279-82.
pallidum, and HSV 1 ( 1) Other No etiology identified 12 ( 15) Total 81 (100) (*)All specimens were analyzed at an independent laboratory using a research prototype multiplex polymerase chain reaction (PCR) assay that can amplify and subsequently detect DNA from H.
To successfully introduce highly multiplexed MDx into the everyday clinical algorithm, new multiplex polymerase chain reaction (PCR) technologies must be developed that can affordably and consistently identify multiple pathogens, resistance mutations carried by pathogens, cancer-associated mutant genes, or host-related gene expressions associated with chronic diseases.
Multiplex polymerase chain reaction for the simultaneous detection and typing of polyomavirus JC, BK and SV40 DNA in clinical samples.
The isolate was subjected to a multiplex polymerase chain reaction followed by an endonuclease cleavage of amplicons by MspI (Invitrogen, Carlsbad, CA, USA) as previously described (7) to detect van gene determinants; this process demonstrated a simultaneous presence of vanC1 and vanB determinants.
The genomic DNA is then converted to single-stranded targets by using multiplex polymerase chain reaction (PCR) amplification and exonuclease digestion.
The presence of mecA and nuc genes was determined by multiplex polymerase chain reaction (PER) using mecA1, mecA2, nuc1, and nuc2 primers (6).
Puncture of the wound released 12 mL of pus; culture of the pus sample yielded an MRSA strain harboring a TSST-1 gene, detected by multiplex polymerase chain reaction as previously described (3).
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