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MyHC are encoded by a multigene family in skeletal muscle of mammalian animals.
Skeletal muscle is characterized by fast and slow muscle based on the expression pattern of MyHC isoforms in muscle fibres (Schiaffino and Reggiani, 1996).
Other serial sections were incubated with a panel of monoclonal antibodies (MAbs) that were specific for MyHC isoforms (Table 1).
(2006) found that the ratio of myosin heavy chain (MyHC) I to other isoforms of the flexor digitorum superficialis muscle and gastrocnemius medialis muscle significantly increased in neonatal IUGR piglets.
Relative distribution of Type I, IIa, IIb, and IIx muscle fibers classified by MyHC phenotypes in RF and SM muscles 4 and 48 weeks after ACL resection are shown in Figures 7 and 8, respectively.
transfected synthetic MyoD mRNA in to hiPSCs for 3 days, which resulted in myogenic differentiation (around 40%) with expression of myogenin and MyHC .
As shown in Figure 4(b), METF treated cells were characterized by a significant rise in principal marker of myotube maturation, MyosinHeavy Chain (MyHC) protein levels with respect to control cells.
We aligned and compared translated sequences of lactate dehydrogenase (LDH-A and LDH-B), cytosolic malate dehydrogenase (MDHc), hemoglobins (Hb-A and Hb-B), actin (ACTA1), and myosin heavy chain (MyHC).
Cell lysates (equal protein loads of 50 [micro]g) were subjected to SDS-PAGE (10-12.5% of gel, depending on the MW of protein) at 150 V, next they were transferred at 100 V for 2 h to polyvinylidene difluoride (PVDF) membrane and immunoblotted with antibodies against phosphorylated and/or nonphosphorylated forms of proteins: actin, NF-[kappa]B (p65), IkB[alpha], STAT1-[alpha], P(Tyr701)-STAT1-[alpha], MyoD, and myogenin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or MyHC IIa (Sigma Aldrich Chemical Co., St.
The differences might be due to the increase in muscle fiber diameter, a decrease in muscle fiber density and the different mRNA expressions of MyHC family genes in the muscles of SJ and JJ.
Contraction of the skeletal muscle requires the function of myosin heavy chain (MyHC).
Additionally, mammalian skeletal muscle consists of 9 myosin heavy chain (MyHC) isoforms.
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- MYH14 variant protein
- MYH9 variant protein