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References in periodicals archive ?
EDC 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide NHS N-Hydroxysuccinimide SA Sodium alginate GA Gelatin REFERENCES
This is followed by adding 2.4 mg/ml of NHS (N-hydroxysuccinimide).
Amouse monoclonal antibody (Clone ID: MV12/1/C2-2/1), specific to JEV was originally provided by Venture Technology, Penang, Malaysia [20, 21], and treated with ethyl(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) for activation.
(2) N-t-Butyloxycarbonyl-Amino Acid N-Hydroxysuccinimide (HONSu) Esters (3a-d)
N-t-Butyloxycarbonyl-glycine (23.73 g, 0.135 mol) and N-hydroxysuccinimide (HONSu) (17.26 g, 0.15 mol) were dissolved in ethyl acetate (410 mL).
To 5 mg of F-21-HS, 200 [micro]L of dimethyl formamide, 200 [micro]L of dioxan, and 100 [micro]L of distilled water containing 10 mg of N-hydroxysuccinimide and 20 mg of 1-ethyl-3-(3-dimethyl-amino-propyl) carbodiimide-HCL were added; the reaction mixture was vortex-mixed and kept overnight at 4 [degrees]C.
The N-hydroxysuccinimide ester of 5b (compound 6b) was prepared using a method analogous to that for compound 6a.
The binding was carried out using 4-(maleimidomethyl)-cyclohexane1-carboxylic acid N-hydroxysuccinimide ester (SMCC), a reagent containing a maleimide group that reacts specifically with--SH groups (13).
HVA, VMA, 3,4-dihydroxyphenylacetic acid, 4-hydroxyphenylacetic acid, 3,4-dihydroxymandelic acid, DA, normetanephrine, metanephrine, epinephrine (adrenaline), vanillic acid, vanillin, phenylacetic acid, dry 1,2dimethoxyethane, ethyldimethylaminopropyl carbodiimide, N-hydroxysuccinimide, diphenyldiazomethane, keyhole limpet hemocyanin (KLH), ovalbumin (OVA, chicken egg), human serum albumin, pyridine, and goat anti-mouse IgG peroxidase were from Sigma Chemical Co.
(3) Nonstandard abbreviations: hGG, human [gamma]-globulin, NHS, N-hydroxysuccinimide; and HSA, human serum albumin.
HVA, VMA, 3-methoxy-4-hydroxyphenylethylene glycol (MHPG), 3-methoxytyramine (MT), ethylene diamine, 3,4-dihydroxymandelic acid (DOMA), vanillin (VAN), 3,4-dihydroxyphenylacetic acid (DOPAC), normetanephrine (NM), 3-methoxytyrosine (3MTyr), dopamine (DA), E, NE, synephrine (Syn), hydroxyphenylacetic acid (HPAA), phenylacetic acid (PAA), tyramine (Tyr), N-hydroxysuccinimide (NHS), 1,3-dicyclohexylcarbodiimide (DCC), N-succinimidyl-4-(N-maleimidomethyl)-cyclohexane-l-carboxylate (SMCC), and N-succinimidyl-S-acetylthioglycolic acid (SATA) were purchased from Sigma (St.
(2) Nonstandard abbreviations: SCT, salmon calcitonin; DELFIA, dissociation and enhancement lanthanide fluoroimmunoassay; IFMA, immunofluorometric assay; SPA, Staphylococcus protein A; and NHS, N-hydroxysuccinimide.