NAD1NADH (Nicotinamide Adenine Dinucleotide) dehydrogenase subunit 1 (gene)
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Por otro lado, el ADN de los ejemplares fue extraido con exito, asi como la amplificacion parcial de los genes mitocondriales cox1 y nad1, produciendo amplicones de ~420 y 480 pares de bases (pb), respectivamente.
Esto se confirmo con el analisis parcial de los genes mitocondriales (cox1 y nad1), llegando a obtener una identidad de mas del 99% con secuencias de F.
van der Weerden, "Identification and mechanism of action of the plant Defensin NaD1 as a new member of the antifungal drug arsenal against Candida albicans," Antimicrobial Agents and Chemotherapy, vol.
Using sequencing with mitochondrial COI and nad1 genes, they verified that the predominant genotype was G1, with the exception of two identified as G2 and G5 (Andresiuk et al.).
DNA was showed (NAD1) dehydrogenase subunit 1 gene amplified from all samples of human hydatid cysts by using specific primers.
PCR amplification and DNA sequencing of the NAD1 locus identified cyst fluid samples as the G8 strain of E.
However, the new cox1 and nad1 sequences are 12% different from those of published V.
Two mtDNA haplotypes were detected that differed by an insertion/deletion of approximately 30 bp in the second intron of nad1. The haplotype frequencies were 0.837 for the longer haplotype and 0.163 for the shorter.
Para el diagnostico molecular, se amplificaron parcialmente los genes mitocondriales: citocromo c oxidasa subunidad 1 (cox1) y NADH deshidrogenasa subunidad 1 (nad1).
Specifically, nuclear contig 269235 contained a contiguous region of 2,008 bp that includes sequences homologous to the mitochondrial genes for nad2, methionyl-tRNA, lysidine synthase, glutamate-tRNA ligase, valine-tRNA ligase, and 12s ribosomal RNA and contig 5819 includes 1,065 bp homologous to nad1. To confirm that the NUMT sequences were not simply errors in assembly, we compared the NUMTs and extant mitochondrial sequences.
With the rejection of the NAD1,5bn offer, Bank Windhoek continues to be a wholly owned Namibian bank in the country.
PCR was performed by using primers for 4 mitochondrial loci: NADH dehydrogenase subunit 1 (nad1) and 2 (nad2), cytochrome b (cob), and cytochrome c oxidase subunit 1 (cox1) (3,4).