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NANOGNorth American Network Operator'S Group
NANOGNorth American Network Operations Group
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Regulation of the pluripotency marker Rex-1 by Nanog and Sox2.
Debemos resaltar que las celulas con inmunofenotipo CD44alta/CD24baja estudiadas en este trabajo fueron obtenidas por inmunoseleccion magnetica, corroborada su pluripotencia por expresar los genes Oct4 y nanog mediante RT-PCR, por la capacidad de inducir la formacion de tumores en ratones con inmunosupresion quimica, eran celulas adherentes, a diferencia de otros estudios que utilizan mamoesferas en suspension (Ghanbari et al., 2016).
Expression of mesenchymal and embryonic stem cell markers by MenSCs and BMSCs Markers MenSCs BMSCs CD34 1.7[+ or -]0.9% 0.92 [+ or -]0.4% CD38 1.4[+ or -]0.7% 1.9 [+ or -]1.3% CD45 1.1[+ or -]0.7% 1.7 [+ or -]0.8% CD133 1.9[+ or -]0.7% 2 1 [+ or -]l.6% CD9 90.8[+ or -]6.7% 92.1 [+ or -]5% CD10 89.4[+ or -]5.7% 40.1 [+ or -]18% CD29 98.7[+ or -]1.26% 94.9 [+ or -]5% CD44 99 [+ or -]0.2% 96.97 [+ or -]3% CD73 99.7[+ or -]0.2% 97.6 [+ or -]1.2% CD105 89.6[+ or -]9.6% 99.7 [+ or -]0% Stro-1 4.7[+ or -]1.4% 8.7 [+ or -]3.4% Oct-4 99.5[+ or -]0% 1.4 [+ or -]l.l% Nanog 2.5[+ or -]04% 7 [+ or -]2.4% SSEA-4 1 8[+ or -]0.8% 78.3 [+ or -]11.4%
NTT Communications Corporation (NTT Com), the global data and IP services arm of Fortune Global 500 telecom leader NTT (TYO: 9432), announced that it is renewing its support to the North American Network Operators' Group (NANOG) as a Diamond Premium Sponsor of its meetings and activities into 2018 and early 2019, including the NANOG 73, 74 and 75 meetings, the company said.
The Oct4 and Sox 2 binding sites are located within the differentially regulated Nanog promoter region and responsible for its transcriptional activity [12].
Both MSC populations maintained expression of the embryonic stem cell marker, Nanog, whilst Oct4 was only highly expressed in CB-MSCs at PD15 with faint expression at PD50 and PD100.
Self-renewal and pluripotency of ESCs are assured by the presence of a few specific factors such as OCT4, SOX2, KLF4, cMYC, NANOG, and LIN28 whose expression is due to a precise chromatin structure derived from epigenetic modifying events that regulate chromatin organization and, consequently, gene expression in all type of cells.
The newer embryonic stem cell transcription factors, OCT3/4 (OCT4), NANOG, and SOX2, play an important role in stem cell growth and differentiation.
The Stowers study suggests that balance between pluripotency and differentiation hinges in part on regulatory communication involving inhibition between Nanog and Hox genes.
qRT-PCR analyses revealed that mRNA levels of Oct4, Nanog, and CK19 were decreased upon induction.
Most of the transcription factors that presented differences between the studied groups (ATF4, CDX2, DDIT3, HSF1, OTX2, POU5F1, XBP1, HAND1, NANOG, NFKB2, SOX2, and SOD2) also have important functions in RNA metabolic processes, regulating the frequency, rate, and extent of chemical reactions involving RNA [33, 35].