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400 [micro]L of the supernatant was mixed with 200 [micro]L phosphate buffer solution consisting of 0.1% of 3-trimethylsilyl propionic-2,2,3,3-d4 acid sodium salt (TSP) as internal standard (adjusted to pH 7.4 using NaOD) and transferred into 5 mm NMR tubes.
Reeglina on inimestel meeles laeva nimi, ilmastikuolud, samuti kaasreisijate naod ja vahel nimedki.
About 150 mg of the freeze-dried material was dissolved in 3 mL of 0.5 m NaOD in a 10 mm NMR tube.
Common solvents used in liquid state [sup.13]C NMR are NaOH and NaOD, and these appear to display no absorptions.