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Gene expression of ECAD (CDH1), SLUG (SNAI2), and NCAM (NCAM1)
Of 15 samples analyzed for NCAM1, 8 showed gene expression (median, 1.14) (Table 2).
Among noninvasive tumors, 5 (55.6%) expressed CDH1, none expressed SNAI2, and 3 (42.9%) expressed NCAM1. Among invasive tumors, 5 (45.5%) expressed CDH1, none expressed SNAI2, and 5 (62.5%) expressed NCAM1 (Table 2).
There was no statistically significant relationship between tumor grade or invasiveness and gene expression of CDH1 (P=0.295), SNAI2 (P=0.485), and NCAM1 (P=0.463).
It is obvious that further studies are needed to fully elucidate the potential roles of NCAM1 in addictive processes under different conditions, including in response to different types of addictive drugs, at different ages and at different parts of the addiction cycle.
Like NCAM1, polysialylation of SYNCAM1 is important to its function, with polysialylation of SYNCAM in the first Ig domain preventing homophilic binding .
Weiss et al., "Haplotype spanning TTC12 and ANKK1, flanked by the DRD2 and NCAM1 loci, is strongly associated to nicotine dependence in two distinct American populations," Human Molecular Genetics, vol.
We used transcript concentrations of marker genes GATA1, RUNX1, SPI1, and NCAM1, commonly involved in leukemogenesis, to study the need for stabilization of BM samples in a clinical setting of disease monitoring.
Q-RT-PCR was performed in a 1-step method with 1 ng total RNA by use of the Quantitect[R] SYBR[R] Green reverse-transcription (RT)-PCR Kit and Quantitect Primer Assays (Qiagen) for the detection of GATA1, RUNX1, NCAM1, and SPI1 and for control gene 18S rRNA.
Differences of [Delta][Delta][C.sub.T], calculated as [Delta][C.sub.T](P2) - [Delta][C.sub.T](P48) for the control gene (18S rRNA), were homogeneous within a tight range: GATA1 0.17 (0.10), RUNX1 0.27 (0.17), NCAM1 0.53 (0.23), and SPI1 0.16 (0.09).
MYOM1 Myomesin 1 2.732 NCAM1 Neural cell adhesion molecule 1 2.657 This gene encodes a cell adhesion protein that is involved in cell-to-cell interactions and cell-matrix interactions during development and differentiation.
Consistent with the in vivo teratoma formation capacity, the cells in the EB could be differentiated into three germ lines in vitro, as demonstrated by the expression of ectoderm (ncam1 and notch), mesoderm (snai1 and snai2), and endoderm ([beta]-catenin and cxcr4) genes (Figure 2).
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