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(2.) NCCLS. Analysis and presentation of cumulative antimicrobial susceptibility test data.
We were unable to identify any previous study on cTnI reference intervals established in the NCCLS standard procedures that included such a large sample of newborn infants.
To compare the precision of the AxSYM and ADVIA Centaur BNP assays, we performed a replication study according to NCCLS guideline EP5-A (6).
The assay linearity of the cortisol determinations in saliva was studied according to the NCCLS EP-6 protocol, in a saliva sample with a low cortisol concentration with added cortisol (Sigma Chemical Co) at a concentration of 240 nmol/L (3).
Ernst's second presentation, "The New CLSI Standards: Important Changes Every Phlebotomist MUST Know" will address recent CLSI (formerly NCCLS) revisions to key standards on specimen collection.
It is formulated at the 1:10 dilution of sodium hypochlorite recommended by the NCCLS, OSHSA, and CDC for inactivating may microorganisms.
Resistance to vancomycin, teicoplanin, gentamicin, and streptomycin was confirmed by the broth dilution method, according to the National Committee for Clinical Laboratory Standards (NCCLS) recommendations (5).
Copies of the standards are available from NCCLS (www.nccls.org), HL7 (www.hl7.org), and IEEE (www.ieee.org).
Sudden differences in units of measure, sensitivity and specificity of the assay, reference ranges, preanalytical variables, even sample type, become big issues for the end-user, and unexpected results focus their attention on "how much better it was in the good old days." For this reason, CLSI (formerly NCCLS) guidelines, (1) CLIA '88 regulations, (2) and prudent practice should lead laboratorians to follow a comprehensive protocol for new-test validation that addresses all of the issues that will be forthcoming when the assay is implemented.
The isolate was found to be resistant to the conventional antityphoid drugs by using modified Kirby-Bauer disk diffusion technique according to the criteria of the National Committee for Clinical Laboratory Standards (NCCLS) (6).
The NCCLS EP14 protocol (8) for evaluating possible matrix effects of processed samples or possible calibrators requires the simultaneous analysis of, preferably, 20 selected fresh patient sera together with the candidate calibrators to be studied by both a particular field method and, preferably, a reference method.
NCCLS document M100-S15, which addresses performance standards for antimicrobial-susceptibility testing, provides lists of potential agents for each organism group based on acceptable in vitro test performance, clinical efficacy, cost, and current consensus recommendations.
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