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Reduction of growth performance in broilers fed LP diets and supplemented with EAA has been reported  irrespective of their environmental condition, and this reduction could be associated with lower levels of specific NEAA such as glycine and serine or glutamic acid, or with anon-specific need for nitrogen .
The adverse effect of LP diets on gut morphometry could be attributed to a decrease in the concentrations of NEAA such as glycine, glutamine and proline, which actively support the epithelial layer and represent a major fraction of intestinal masons and digestive secretions .
May 5 (Sat) 20:15 GMT + 1, Slovakia - Czech Republic; 2:3 in overtime (1:0, 1:1, 0:0 - 0:1), Scores: Michal KriA!tof and Andrej Sekera for Slovakia and Dominik Kubalik, Martin NeAas (2 times) for the Czech Republic
Cells from passage number 70-82 were maintained in 75 [cm.sup.2] plastic culture flasks in 10 ml of Dulbecco's Modified Eagle's Medium, supplemented with 10% FBS Hyclone[R], 1% NEAA and 1% penicillin/streptomycin (100x), cultivated in a humidified incubator at 37[degrees]C, 5% C[O.sub.2] until 70-90% confluency was achieved.
Abbreviations A apical B basolateral BBB blood-brain barrier BCEC brain capillary endothelial cells BCSFB blood cerebrospinal fluid barrier CNS central nervous system DAPI 4',6-diamidin-2-phenylindol DMSO dimethylsulfoxide EDTA ethylenediaminetetraacetic acid EVOM epithelial voltohm meter FBS fetal bovine serum HBSS Hank's balanced salt solution MIT 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide NEAA non-essential amino acids NMDA N-methyl- d -aspartate [P.sub.app] apparent permeability coefficient [cm/s] PBS phosphate buffered saline SE hydroethanolic saffron extract SGF simulated gastric fluids SIF simulated intestinal fluids TFA trifluoroacetic acid TEER transepithelial electrical resistance Acknowledgment
The scandal - which forced NeAas to resign - involved widescale spying by the intelligence services, bribery, and a byzantine plot that was remarkable even by the standards of Czech public life.
Apart from the colourful detail that surrounded the government's demise, it was significant for the reason that NeAas was leader of the Civic Democrats, the main centre right party and the equivalent of the Conservatives in Britain.
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