NENSPNew England Newborn Screening Program (University of Massachusetts)
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In addition to testing newborns in Massachusetts, the NENSP tests newborn specimens from Maine, New Hampshire, Vermont, and Rhode Island.
All specimens and controls tested by the NENSP were collected or prepared on S&S Grade 903 filter paper (Schleicher and Schuell).
Two laboratories analyzed the DNA: one laboratory (Amino Acid Lab, Massachusetts General) used the method of Tanaka (15), and the second (NENSP) used a Chelex procedure [the 3.2-mm blood spot was washed by shaking for 30 min in 300 [micro]L of wash buffer (1 X phosphate-buffered saline and 5 mL/L Triton X-100 by volume)]; after removal of the buffer, 120 [micro]L of Chelex Solution (10% by volume) was added followed by a 30-min incubation at 100[degrees]C.