NEPHGENon-Equilibrium pH Gradient Electrophoresis
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Abbreviations: COR, cold-regulated; LMWBP, low molecular weight basic peptides; [LT.sub.50], lethal temperature for 50% of the plants; mRNA, messenger RNA; NEPHGE, non-equilibrium pH gradient electrophoresis; 2D-PAGE, two-dimensional polyacrylamide gel electrophoresis.
In vitro translation products were separated by 2D-PAGE with the first dimension performed by electrofocalization in a non-equilibrium pH gradient (NEPHGE; O'Farrell et al., 1977) in 2% pH 3.5-10 Ampholines (Pharmacia).