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Fujihara, "Expressed nifH genes of endophytic bacteria detected in field-grown sweet potatoes (Ipomoea batatas L.)," Microbes and Environments, vol.
For the nifH gene amplification, the first PCR was carried out with the forward primer FGPH19 and the reverse primer POLR (Table S1) [21, 22], generating a product of 429 bp.
The generated amplicons were used in a second reaction for the nifH gene, using the primers PolF-GC (5'- GCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGC- CCCTGCGAYCCSAARGCBGACTC-3') and AQER (5'-ACGATGTAGATYTC CTG-3').
coli strain JM109(DE3), carrying plasmids pRT22 (Klebsiella pneumoniae nifH::lacZ) and pET-29a with different nifA mutations, was analyzed as described previously (9).
Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil.
Standard curves containing known copy numbers of each gene were generated using serial dilutions of linearised plasmids containing either a cloned nifH gene (from pure cultures of Azospirillum sp.) or amoA gene (from pure cultures of Nitrosomonas sp.); data were linear for [10.sup.1]-[10.sup.6] gene copies.
The DSR genes abundant in 8F were dsrA and dsrB; the methanol-oxidation category was dominated by pmoA and mmoA from methanotrophs and nitrogen fixation genes categorized as nifH from nitrogen-fixing bacteria (Table S4).
The nifH gene, a gene associated with the fixation of atmospheric nitrogen, has also been found in this bacterium (GenBank accession JF736510) and it has been experimentally shown that this strain is capable of fixing nitrogen in vitro suggesting possible nitrogen fixing attributes in its natural environment, the ant body cavity .
Further research focusing on nifH gene diversity in two species of Montipora (Olson et al., 2009) suggests that nitrogen-fixing bacteria in corals are not limited to cyanobacteria but also belong to taxa representing the [alpha]-, [BETA]-, [gamma]-, and [delta]-proteobacterial classes (Olson et al., 2009).
Genetic characterization of Cylindrospermopsis raciborskii (Cyanobacteria) isolates from geographic origins based on nifH and cpcBA-IGS nucleotide sequence analysis.
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- Nifontova, Rufina
- Nifontova, Rufina Dmitrievna