NLDL

AcronymDefinition
NLDLNative Low-Density Lipoprotein
NLDLNewfoundland and Labrador Defense League (Canada)
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References in periodicals archive ?
For subculture, the cells on a Petri dish (6 x [10.sup.4] cells; [empty set], 35 mm dish) were subcultured twice concomitantly with nLDL treatment.
Young HUVECs (PDL, 12-15) on a Petri dish (1.0 x [10.sup.5] cells; [empty set], 10 cm dish) were subcultured and treated with various concentrations of nLDL (0, 2, 5, or 10 [micro]g protein/mL) for up to 9 days.
Briefly, the cells on a Petri dish (1.0 x [10.sup.5] cells; [empty set], 10 cm dish) were subcultured and treated with nLDL (0, 2, 5, or 10 [micro]g protein/mL) for up to 9 days.
Protein concentrations of cell lysates were determined by the method of Lowry [29]and those of nLDL and oxLDL were obtained using Peterson's modification of Lowry's method [30].
The compositions (protein, total cholesterol, and TBARS content) of the nLDL and oxLDL preparations used are shown in Table 3.No significant differences were found in the protein or total cholesterol content of nLDL as compared to oxLDL.
THP-1 macrophages were incubated with or without nLDL or oxLDL (50 [micro]L protein/mL) for 8h or 24 h, and lysates were then used to determine the abundance of transcripts for Myo6, Dab2, AP-2[alpha]2, and GIPC1 by a combination of RT-qPCR and immunoblotting (the latter for Myo6 and Dab2).
For characterization of the obtained antibodies, we performed several forms of Western blotting: (a) dot-blot with pure cLDL and nLDL antigens; (b) Western blotting after nondenaturing electrophoresis in 0.5% agarose gels; and (c) Western blotting with cLDL, nLDL, and ApoB after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in 4-20% gradient gels.
Briefly, the wells in a 96-well plate were precoated with either cLDL or nLDL solution (50 [micro]L of 5 mg/L in PBS-E per well) overnight at 4[degrees]C.
All patient sera were diluted 1:200 and 1:1000 in blocking buffer for the cLDL and nLDL measurement assays, respectively.
Briefly, the cells on a Petri dish (1.0 x [10.sup.5] cells; 0, 10 cm dish) were subcultured and treated with nLDL (0, 2, 5, or 10 [micro]g protein/mL) for up to 9 days.
Young HUVECs on a culture dish (1.0 x [10.sup.5] cells; [phi], 10 cm dish) were subcultured and treated with various concentrations of nLDL (0, 2, 5, or 10 [micro]g protein/mL) for up to 9 days.
The cells were treated with low concentrations of nLDL (0, 2, 5, and 10 pg protein/mL) and cellular senescence was analyzed by quantitative assay (Figure 2(a)) as well as staining (Figure 2(b)) of SA-[beta]-Gal activity in a subculture system for up to 9 days.