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The cultural filtrate obtained after centrifugation of the fermentation broth was subjected to ultra filtration using Pellicon Cassette system with a membrane PLGC Cassette 10.000 NMWL, low protein-binding.
Ultrafiltration with 30,000 and 10,000 NMWL membrane cassettes resulted in the recovery of 77 per cent protease activity from the culture filtrate and 20-fold reduction in volume.
We quantified levels of antioxidant enzymes in the BAL fluid of exposed and control subjects, which had been previously concentrated 10-fold by ultra-filtration using the Ultrafree-CL, NMWL 5,000, centrifugal filter unit (Millipore, Billerica, MA, USA).
Unincorporated primers, dNTPs and the small XbaI fragment were removed by dialysis with a Millipore Ultrafree-MC 30 000 NMWL filter unit (Millipore Corp., Bedford, MA).
Single-stranded amplification products were purified with Millipore Ultrafree-MC (NMWL: 30,000) filters, and sequenced directly by the dideoxynucleotide chain-termination method (Sanger et al.
After overnight growth, Candida cell suspensions were centrifuged (6,000 x g for 8 min), and the supernatant was collected, then concentrated by using a Centricon 10,000 NMWL filter centrifuge (Millipore Corp., Billerica, MA).
PCR products were desalted using Ultrafree R-MC (30 000 NMWL) filter units (Millipore Corp.).
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