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NOS2Nitric Oxide Synthase 2
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B-C) FBXW7[alpha] siRNA plasmid or the control vector, were introduced into RAW264.7 for 36h, and then the cells were co-cultured with colon-26 cells for 24h, B) In parallel, total RNA was isolated from co-cultured macrophage for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of cyclooxigenase (COX)-2 and nitric oxide synthase 2 (NOS2); C) The cellfree supernatant was collected and analyzed by ELISA against IL6, IL12p40, and tumor necrosis factor-[alpha] (TNF[alpha]).
In our study, the only significant effects of RBC transfusion on pulmonary inflammation were the modifications on pulmonary gene expression of NOS2 and IL-21.
Strikingly, the M1 markers Cxcl9 and Nos2 and M2 marker Tgm2 were significantly upregulated in the macrophage populations of tumor-bearing mice (p = 0.00052 (Figure 2(b)), 4.2E-07 (Figure 2(c)), and 0.0025 (Figure 2(d)), respectively), displaying no clear transition in terms of polarization.
No difference was found in PGE2, TGF[beta]1, Nos2, and IDO1 between both groups (Figures 2(a)-2(d)).
Moreover, we compute the values CN([P.sub.i]) for numerical order scales NOS 1, NOS2, and NOS3 described in Table 5.
Dermatomyositis with or without anti-melanoma differentiation-associated gene 5 antibodies: Common interferon signature but distinct NOS2 expression.
QRT-PCR analysis indicated the transcription of the selected lncRNAs: RP11-517C16.2-001, FR271872, LOC283352, RP11-401E9.3, FGFR3P, XXbac-BPG16N22.5, and mRNAs: collagen 19a1 (COL19A1), nitric oxide synthase 2 (NOS2), chromosome 13 open reading frame 15 (C13orf15), FBJ murine osteosarcoma viral oncogene homolog (FOS), ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) (FCN2), serine peptidase inhibitor, Kunitz type 1 (SPINT1), placenta-specific 8 (PLAC8), E2F transcription factor 1 (E2F1), and baculoviral IAP repeat containing 5 (BIRC5), perfectly correlated to microarray results.
Polychlorinated biphenyls (PCBs) congener 126 and congener 153 modify the following inflammation related genes: AHR, CXCL2, HMOX1, IFNG, IL6, PTGS2, SOD2, and TNF; AHR, CXCL8, HMOX1, IL1B, IL6, MMP9, NOS2, NOS3, PARP1, PTGS2, and TNF; and AHR, IFNG, IL1B, PARP1, PTGS2, and TNF.
Since the transcriptions of ARG1, IDO2, inducible nitric oxide synthetase (NOS2), Ptgs2, Ptger4, and NOX1 are all regulated by the coactivators CEBPA/B, which also function in this capacity with PPAR[delta], this suggests a mechanism whereby PPAR[delta] may control adaptive immunity metabolically within the tumor microenvironment.
Subsequently, the PVDF membranes were probed with primary antibodies against NOS2 (1: 1000, CST, US), CD206 (1: 1000, CST, US), TNF-[alpha] (1: 1000, CST, US), and PDGF (1: 1000, Santa Cruz, CA, USA) at 4[degrees]C overnight.
Hara et al., "NOS2 polymorphisms associated with the susceptibility to pulmonary arterial hypertension with systemic sclerosis: contribution to the transcriptional activity," Arthritis Research & Therapy, vol.
After blocking with 10% nonfat milk in PBS-Tween20, membranes were incubated with antibodies IBA1, GAPDH, p65, histone H3, NOS2, and NOX2 (Abcam China, Shanghai, China).